Veterinaria Medika
ISSN 1979-1305
Vol. 5 / No. 3 / Published : 2012-11
Order : 12, and page :219 - 226
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Original Article :
Insulin like growth factor-i complex sebagai alternatif antioksidan pada media pendewasaan spermatozoa kambing
Author :
- Triana. I.N*1
- Susilowati, Suherni*2
- Yuliati, G.A*3
- Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
- Fakultas Kedokteran Hewan Universitas Airlangga
Abstract :
One of the protein in the seminal plasm of goat is Insulin Like Growth Factor–I (IGF-I) Complex. IGF-I Complex has molecular weigth 150,288 kDa which consisted of three protein molecules that were IGFI (subunit α), IGFBP (subunit β)and ALS (Acid Label Subunit) (subunit ¥) The research was done into two phases. First phase concerned with explorative to identify and isolation of IGF-I Complex and second phasewas to supplementation of IGF-I into capatitation medium of goat sperm. The aim of this research was to decreased of MDA concentration. The benefit of this research was to increased of the sperm quality so thatcan used for in vitro fertilitation. Analysis with varian showed significant different (P<0,05) to MDA concentration. From results above can be concluded that potein of IGF-I Complex can decreased of MDA concentration in sperm capacitation medium.
Keyword :
IGF-I Complex, MDA, Capacitation,
References :
Agarwal, A; RA. Saleh and MA Bedalwy,(2003) Role of Sperm Parameter to level of Reac tive Oxpecies in Semen Specimens. . : Urol. 152 : 107-110
Balboni, G.C, B.G. Vannelli, T. Barni, C.Orlando, A. Natali and M. Serio,,(1988) Insulin- like growth factor–I (IGF-I) and IGF-I receptor in human testes : an immunohistochemical study. . : Ferti Steril. 49(4) : 666-9.
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