UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 9 / No. 3 / Published : 2016-11

Order : 9, and page :61 - 66

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Original Article :

Kualitas spermatozoa domba merino pada sisi anoda hasil pemisahan dengan teknik ess ( electric separating sperm)

Author :

  1. Amung Logam Saputro*1
  2. Herry Agoes Hermadi*2
  3. Sri Mumpuni Sosiawati*3
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This study is one of new applied science in artificial insemination in sheep inwhich sperm cells produced by males can be separated in chromosom X and Y of sex.In this study used the ESS (Electric Separating Sperm) technique as separation methods.Researchers used a new tool to separate sperm cells by using an electric tool that electrified1.5 volts, which compared to the level of effectivenes with different time is 0 minutes,3 minutes, 7 minutes, and 10 minutes. Data analysis using LSD forwaded anova with(Turkey) 5% by using 5 replicates per treatment. P0 in this cntrol (0 min), P1 (3 minutes),P2 (7 minutes), P3 (10 minutes). Data results showed that motility of P052,40 + 1,661 a,group of P151,40 + 2,337 a, group of P250,40 + 1,778 a, and group of P3 48,00 + 1,000a. Cell viability of P0 64,40 + 2,315 a , group of P1 60,20 + 2,653 ab, group of P2 56,00 +2,302 ab, and group of P3 54,20 + 2,746 b . and abnormalities of P0 4,60 + 1,435 a, groupof P1 7,00 + 1,304 ab, group of P2 11,00 + 2,345 ab, and group of P3 11,40 + 1,030 b.The results showed that the quaity of spermatozoa in microscopic oservation cells test ofmotility in all treatment groups showed no significant differentces, whereas the viabilityand abnormality observations indicate that the results between the P1 and P2 control donot have significant differences but significantly different P3

Keyword :

Separating Spermatozoa , ESS (Electric Separating Sperm), Semen quality,


References :

Bearden, H.J. and J.W. Fuquay. ,(1992) Applied Animal Reproduction 3rd edition. 164-172, 223-224. : New Jersey : Prentice Hall, Englewood clifts,.

Hafs, H.D and L.D. Boyd..,(1986) Galvanic Separation of X and Y Chromosome Bearing Spermatozoa. In SE Hafez (ed). Reproduction in Farm Animals. 5th edition. 498-500. : By Lea and Febiger. Philadelphia

Salisbury, G.W and N.L. Vandermark. .,(1985) Fisiologi dan Inseminasi Buatan pada Sapi (Physiology and Artificial Insemination of Cattle). Diterjemahkan oleh Januar. 319,334-339,312-343. : Gajah Mada University Press Yogyakarta.





Archive Article

Cover Media Content

Volume : 9 / No. : 3 / Pub. : 2016-11
  1. The Effect Of Spirulina Platensis Extract Immersion As Immunostimulant To Intestine Histopathology Of Aeromonas Hydrophila Infected Gouramy (osphronemus Gouramy)
  2. Effect Of Vitamin E And Vitamin C Combination As Preventive Measure On The Leydig Cell Number In Mice (mus Musculus) Treated With Borax
  3. The Influence Of Beta Carotene To Live Fetus Percentage In Mice (mus Musculus) That Exposured By Cigarette Smoke
  4. Effects Of Vitamin C And Vitamin E Combinationas Prevention On The Liver Histopathological In Mice (mus Musculus) Treated With Borax
  5. Effect Of Moringa Oleifera Leaf Extract On The Percentage Of Necrotic Purkinje Cells In Mice (mus Musculus) Induced By Methylmercury
  6. Hepatoprotective Effect Of Andrographis Paniculata Ness. Leaves Extract Towards Mice (mus Musculus L.) Liver Histopathology Which Exposured Bylead Acetate Orally
  7. The Effect Of Mimosa Pudica Root Extract To Histopathological Representation Of Rattus Norvegicus Liver Injected With Naja Sputatrix Venom
  8. Kualitas Spermatozoa Domba Merino Pada Sisi Anoda Hasil Pemisahan Dengan Teknik Ess ( Electric Separating Sperm)
  9. Effectivity Of Rat Bone Marrow Mesenchymal Stem Cell Therapy On Rattus Norvegicus Exposed To Carbon Black As Observed From Caspase-3 Expression
  10. Larvaside Effectiveness Of Srikaya Seed Extract (annona Squamosa L.) Against Mortality Of Anopheles Aconitus Larvae
  11. Therapeutic Effect Rat Bone Marrow Mesenchymal Stem Cell (rbm-msc) At The White Rat Endothelial Cells Induced Carbon Black To Total Hofbauer Cells
  12. Identification Of Bacteri Methicillin Resistent Staphylococcus Aureus And Methicillin Sensitive Staphylococcus Aureus From Dog Nasal Mucous
  13. Expression Of Cytochrome C And Capacitation Reaction Frozen Semen Fresian Holstein Bulls (fh) After Thawing In Different Temperature And Duration In Diluent Milk Egg Yolk
  14. Detection Of Bacterial Contamination Amount At Meat Which Is Soaked In Chitosan Solution With Total Plate Count Method
  15. The Effect Of Electrical Stunning On The Ph Values Musculus Fibularis Longus Broiler Chicken

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