UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 3 / Published : 2015-11

Order : 2, and page :251 - 262

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Original Article :

Lactoferrin effect on biofilm formation of klebsiella pneumoniae isolated from mastitis cases of dairy cows

Author :

  1. Ratna Sari Yudaningrum*1
  2. Anwar Ma’ruf*2
  3. Nenny Harijani*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

Klebsiella pneumoniae is one of the main infectious agents responsible for causing mastitis. The response of Klebsiella mastitis to treatment is poor. We speculate that one of the reasons behind the prevalence of K. pneumoniae infections is its ability to form biofilms. The objectives of this study were to determine in vitro biofilm formation and we also observed the effect lactoferrin in the biofilm production. Tweleve strains of K. pneumoniae isolated from chronic mastitic cows, were tested by microtiter plate (MP) and Phase Contrast Microscopy (PCM). The MP methods were used to observed the effect of lactoferrin (LF) in the biofilm production. K. pneumoniae ATCC13833, was used as a control in bacterial identification and biofilm formation. The LF were used in three different concentration 0.1 mg/ml (LF0.1), 0.5 mg/ml (LF0.5) dan 0.85 mg/ml (LF0.85). There are 12 strain displayed a biofilm-phenotype when tested by MP including the ATCC and one strain not forming biofilm. The strains were further classified as weak (41.67 %) and strong (50%) biofilm formers. LF0.1 is enhanced the forming of biofilm in vitro as for LF0.5 and LF0.85 diminished biofilm formation of K. pneumoniae in vitro.

Keyword :

K. pneumoniae, Biofilm formation, Lactoferrin,


References :

Adlerova, L., A. Bartoskova dan M. Faldyna,(2008) Lactoferrin : a Review 9 : 457–468 : Veterinari Medicina

Balestrino, D., J. A. J. Haagensen, C. Rich and C. Forestier,(2005) Characterization of Type 2 Quorum Sensing in Klebsiella pneumoniae and Relatioship with Biofilm Formation. 187 (8) : 2870-2880 : Journal of Bacteriology

Johnson, J. G., C. N. Murphy, Jean Sippy, Tylor, J. Johnson dan Steven Clegg,(2011) Type 3 Fimbriae and Biofilm Formation are Regulated by the Transcriptional Regulators MrkHI in Klebsiella pneumoniae 193 (14): 3453-3460 : Journal of Bakteriology





Archive Article

Cover Media Content

Volume : 8 / No. : 3 / Pub. : 2015-11
  1. Genotyping Viral Nervous Necrosis (vnn) In Groupers In East Java And West Nusa Tenggara
  2. Lactoferrin Effect On Biofilm Formation Of Klebsiella Pneumoniae Isolated From Mastitis Cases Of Dairy Cows
  3. Effect Of Insulin Transferrin Selenium (its) Supplementation In Maturation Medium Tcm-199 To Bovine Oocyte Diameter
  4. Supplementation Of Insulin Transferrin Selenium On In Vitro Maturation Cumulus Oocyte Complex (coc) Against To Mitogen Activated Protein Kinase (mapk) Expression And Expanded Cumulus Mass
  5. Adaptation Capability And Antigen Stability Of Newcastle Disease Virus Local Isolate On Hela Cell
  6. Comparison Of Antibody Titre Of Anti Brucella Abortus Using Cft And Ielisa To Cattle With Positive Rbt
  7. Potency Of Apple Vinegar As Alternatif Therapy Against Wound Healing Time Of Staphylococcus Aureus Infection On Mice
  8. Suplementation Of Specific Protein Membrane Spermatozoa In Freezing Medium To Improvement Efforts A Succes Cattle Frozen Semen Quality Post Thawing
  9. Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Total Leukocytes And Leukocytes Differential Count On Rat After Infected By Salmonella Typhimurium
  10. The Potential Of Outer Membrane Protein Brucella Abortus Local Isolate As Brucellosis Vaccine Development In Indonesia
  11. The Using Of Whole Serum Pmsg (pregnat Mare Serum Gonadotropin) With Sepadex Extracted For Increasing Estrus Induction Of Ettawa Goat
  12. Toxicity Test Of Singawalang (petiveria Alliaceae) Leaves Extract To The Hypertrophy Of Male White Mice (mus Musculus) Cardiac Muscle
  13. Effect Of Benzo(a)pyrene Injection To The Initiation Of Mammae Cancer At Mice

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