UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 2 / Published : 2011-07

Order : 15, and page :153 - 156

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Original Article :

Mengukur kadar protein fertility associated antigen (faa) pada semen sapi menggunakan qubit fluorometer

Author :

  1. Sardjito, Trilas*1
  2. Megawati, A.A*2
  3. Suprayogi, T.W*3
  4. Tjitro Handayani*4
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. PPDH Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Fertility Associated Antigen (FAA) is a protein on sperm bull which helpful to increase pregnancy rate. The present of this protein specifically can increase bull fertility. This research aimed to measure FAA on bull sperm counted by Qubit Fluorometer. Sample used are semen from 4 bulls which are 2 Simmental, 1 Friessian Holstein, and 1 Limousine. Semen obtained from frozen semen laboratory Teaching Farm ofAirlangga University. Semen is accommodated using artificial vagina. Then do the macroscopic (volume, scent, pH, color, and consistency) and microscopic (motility, viability, and consentration) examination.Sample then tested result in protein FAA. From some protein, 31 kDa show up and that protein which identified as FAA after it is reacted with monoclonal antibody anti-FAA. After that the level is counted by Qubit Fluorometer. The result is 31,0; 30,1; 31,6; and 50,9 (µg/mL elusi sample). This research show thatFAA on each bull is different.

Keyword :

FAA, Semen, Qubit Fluorometer,


References :

Lenz. R. W., H. Zhang, J. N. Oyarzo, M. E. Bellin, and R.L.Ax.,(2000) Bovine Fertility-Associated Antigen (FAA) and a Recombinant Segment of FAA Improve Sperm Function. . : SSR, Abstract 80

Hafez,E.S.E,(2000) Reproduction in Farm Animal. 7 th ed. Philladelphia : Lippicott Williams dan Wilkins

McCauley, T. C., M. E. Bellin, Z. Huanmin, and R. L. Ax,(1999) Purificat ion and Characterization of Fertility-Associated Ant igen (FAA) in Bovine Seminal Fluid United States : Mol. Reprod. and Dev. 54 (1999): 145– 153





Archive Article

Cover Media Content

Volume : 4 / No. : 2 / Pub. : 2011-07
  1. The Effect Of Tyrosine Kinases Addition Into The Egg Yolk Skim And Egg Yolk Tris Extender The Motility And Live Spermatozoa Percentages Of The Holstein Friesian Post Thawing
  2. Avian Influenza H5 Virus Excretion Post Challenge Test Of Chickens Immunized With Avian Influenza (h5n1) Virus Indonesian Strain And Commercial Heterologous Vaccine
  3. Reepithelialization And Ck 16 Expression On Superficial-thickness Wound With Topical Hyaluronate And Freeze-dried Amniotic Membrane Treatment
  4. The Effects Of Various Concentration Propanediol Cryoprotectan At Vitrification Process To Viability Of Mice Embryos Post Thawing
  5. Activity Test Of Gynura Procumbens Leaves Extract As Antiangiogenic On Chick Embryo Chorioallantoic Membrane Induced By Basic Fibroblast Growth Factor (bfgf)
  6. Efect Of Centrifugation Of Goat Semen To Free Radical Concentrate On Washing Medium
  7. Isolation Identification And Sensitivity Test Of Bacillus Subtilis From Shrimp Culture Ponds To Antimicrobial Agents
  8. The Differences In Several Parameters Of The Femur Bone Related To The Biomechanics Of The Bucks And Doe
  9. The Influence Of Temperature On Meat Decay One Of Beef Sold The Traditional Market
  10. Antibody Response And Protectivity In Chickens After Vaccination With Nd Lv12 Active Vaccine
  11. Concentration Of Pregnancy Associated Glycoprotein (pag) Of Milk Of Dairy Cows At 21 And 28 Days Post Artificial Insemination
  12. The Increase Of Activated Goblet Cells In The Duodenum Of Broiler Exposed By Heat Stress
  13. (z)-9- Heptacosene Sex Pheromone Level Production On Cuticular Hydrocarbon Field Of Strain Musca Domestica
  14. Antibacterial Potency Of Honey And Noni Juice (morinda Citrifolia L.) To The Number Of Staphylococcus Aureus On Chicken Carcasses
  15. Mengukur Kadar Protein Fertility Associated Antigen (faa) Pada Semen Sapi Menggunakan Qubit Fluorometer

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