UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 4 / No. 3 / Published : 2011-11

Order : 1, and page :157 - 164

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Original Article :

Potential carbofuran induced oxidative stress, decreased choline esterase and increased brain cell embryonal death

Author :

  1. Epy Muhammad Luqman*1
  2. Ari Gunawan*2
  3. Harjanto*3
  4. I Ketut Sudiana*4
  5. Widjiati*5
  1. Dosen Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran
  3. Dosen Fakultas Kedokteran Hewan
  4. Dosen Fakultas Kedokteran
  5. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this research was to determine the potential of carbofuran insecticide in inducing oxidative stress, lowers choline esterase (CHE) and increases cell death during embryonic brain development. Increased cell death during embryonic brain development leads to reduced fetal reflexes and motor function caused carbofuran contaminated during pregnancy. This laboratory experimental studies using 120 mice and carbofuran given by gavage with a fraction of the LD50 dose in mice pregnancy 6-15 days age. At the pregnancy 17 days age, mice were sacrificed and the fetal brain was taken to measure of fetal malonidialdehid (MDA) and choline esterase (CHE) levels, and made preparations to observe the expression of P53 and apoptotic cells undergoing Apopteg Apoptosis Detection Kit. The measurement results were presented in a descriptive variable. Conclusion, LD50 doses of carbofuran insecticides have been found in mice was 0.5 mg, choline esterase and total protein levels decreased as the dose given. Malonidialdehid levels (MDA) of fetal brain increased as the dose given. There were indications of a relationship between the expression of P53 by the number of apoptotic cells and so it is possible apoptotic cell death through the intrinsic pathway.

Keyword :

Carbofuran, Oxidative Stress, Choline Esterase, Cell Death, Fetal Brain,


References :

Eskenazi B, Rosas LG, Marks AR, Dradman A, Harley K, Holland N, Johnson C, Fenster L and Barr DB,(2008) Pesticide Toxicity and The Developing Brain . : Basic & Clinical Pharm & Toxicol. 102: 228-236

Friedlander RM,(2003) Mechanisms of disease Apoptosis and Caspases in Neuro-degenerative Diseases . : The new england journal of medicine. 1365-1375

Otieno PO, JO Lalah, M Virani, IO Jondiko and Schramm,(2010) Carbofuran and its toxic metabolites provide forensic evidence for furadan exposure in Vulture (Gyps africanus)in Kenya. . : Bull Environ Contam Toxicol. 84 : 536-544.





Archive Article

Cover Media Content

Volume : 4 / No. : 3 / Pub. : 2011-11
  1. Potential Carbofuran Induced Oxidative Stress, Decreased Choline Esterase And Increased Brain Cell Embryonal Death
  2. Crossbreds Entog And Duck Artificial Insemination Technology Of Diluted And Doses For Fertibily
  3. Pengaruh Pemberian Alkaloid Daun Jarong (achyranthes Aspera Linn) Pada Mencit Yang Terkena Kanker Mammae Terhadap Gambaran Hitung Jenis Leukosit
  4. Motilitas, Persentase Hidup Dan Keutuhan Membran Spermatozoa Domba Ekor Gemuk Post Thawing Dalam Tiga Macam Diluter
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