Veterinaria Medika
ISSN 1979-1305
Vol. 5 / No. 1 / Published : 2012-02
Order : 7, and page :27 - 36
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Original Article :
Supplement of acrosin to the post thawing sperm of half-breed of etawa goat (pe) towards increasing potency of spermatozoa
Author :
- Utomo, Budi*1
- Fakultas Kedokteran Hewan Universitas Airlangga
Abstract :
The development of goats population in Indonesia has not reached satisfactory condition, in East Java in the year 2007 the population of goat decreased for about 3,24 % (Anonimous, 2007). The governmenthas been trying to solve the problem concerning the decreas of goat population by using artificial insemination, however this effort has not been resulted in satisfactory condition. One of the main factor of the decreasing goat population is the reproduction problem, especially fertilization failure, in which spermcell has not capable to fertilize the ovum. The failure of sperm cell penetration is caused by the reducing enzyme potency in the spermatozoa, especially acrosin enzyme that has function of the zona pelusida in theovum. In Indonesia, research about role and acrosin function in the fertilization, especially penetration on thezona pelusida in ovum of goats has not been reported yet. The general aim of the research was to determinethe role of acrosin towards the biological potency of spermatozoa to penetrate the zona pelusida. The specific aim of this research was to determine effect of acrosin against the quality and the penetration of spermatozoa to the zona pelusida. The experiment was goat spermatozoa supplemented with acrosin dosages 0; 3.0; 4.5 and 6.0 µg toward fertilization in vitro. The result of fertility test with acrosin supplemented 0; 3.0; 4.5; and6.0 µg the rate of cleavage of the oocyte was 40; 60; 60; and 0% respectively. In conclusion, dosage acrosin supplement 3.0 µg for 30 minutes was enough to induce cleavage.
Keyword :
Acrosin, Penetration of zona pelusida, Biological Potency,
References :
Hermadi, H.A,(2009) Bioaktivitas Human Menopause Gonadotrophin (hMG) dan hMG Terdeglikolisasi Dari Urin Perempuan Pascamenopause Terhadap Terbentuknya Cleavage Embrio Sapi Secara In-Vitro Surabaya : Disertasi. Program Pascasarjana Universitas Airlangga
Duan, H., X. Zhang, Y. Li, M. Gao, L. Den and H. Feng,(2007) Study on the relationship of sperm acrosin activity with fertilization rate during in-vitro fertilization. China : Chinese Journal of Andrology Pages 4246.
Adel A. Zalata, H. Ashraf Ahmed and H. Frank Comhaire,(2004) Relationship between acrosin activity of human spermatozoa and oxidative stress Shanghai : Asian J. Androl.: 313-318
Archive Article
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 Volume : 5 / No. : 1 / Pub. : 2012-02 |
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