UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 8 / No. 2 / Published : 2015-07

Order : 9, and page :169 - 178

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Original Article :

The effectiveness of giving haemopoetic stem cells in the pregnant mice (mus musculus) exposed to plumbum towards the decreasing number of necrocis cells and expression of caspase-3 in placenta

Author :

  1. Kamal Musthofa*1
  2. Widjiati*2
  3. Pudji Srianto*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

The aim of this study to observe the effectiveness of Haemopoitic Stem Cell in pregnant mice exposed to plumbum, and the observed cases are the numbers of necrosis cells and caspase-3 expression in the placenta. Haemopoetic Stem Cells was expected to able to reduce the number of necrosis cells and was abled to reduce the expression of caspase- 3. This study used a randomized design, and the required samples in this study were 40 female mice and 40 male mice (Mus musculus). Each treatment utilized 10 male mice and 10 female mice with details as follow, (1) first treatment group (T1): pregnant mice were given a dose of plumbum 1/12 LD50 (54.42 mg / ml) during gestation days 9-15. (2) second treatment group (T2): pregnant mice were given a dose of plumbum 1/16 LD50 (40.75 mg / ml) during gestation days 9-15. (3) Third treatment group (T3): Plumbum pregnant mice were given a dose of 1/12 LD50 (54.42 mg / ml) during gestation days 9-15 and on day 9 Hemopoetic Stem Cell 1x107 was given intravenously. (4) fourth treatment group (T4): pregnant mice were given a dose of Plumbum 1/16 LD50 (40.75 mg / ml) during gestation days 9-15 and on day 9 Hemopoetic Stem Cell 1x107 was given intravenously. The results of the expression of caspase-3 between treatments are significant, especially the fourth treatment group (T4). Of all the treatments (T1, T2, T3) when compared to treatment P4 shows a significantly different result. T4 was treated by giving Hemopoetic Stem Cells on day 9 which coincided with the first day of plumbum exposure. Intended stem cells function properly because the homing process is good enough for stem cells.

Keyword :

Plumbum, Hemopoetic Stem Cell, Caspase-3, Trophoblast Cell,


References :

Hougaard, K.S., K.A. Jensen., P. Nordly., C. Taxvig., U. Vogel1., A. T. Saber and H. Wallin,(2008) Effects of Prenatal Exposure to Diesel Exhaust Particles on Postnatal Development, Behavior, Genotoxicity and Inflammation in Mice 5:3 : Particle and Fibre Toxicology





Archive Article

Cover Media Content

Volume : 8 / No. : 2 / Pub. : 2015-07
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  2. The Effects Of Insulin Transferin Selenium (its) Supplementation On Embryo Freezing Media At Morula Stage Towards The Percentage Of Embryonic Development At Blastula Stage
  3. The Liver Histopathology Of Mice (mus Musculus) Cause Exposure Carbofuran Insectiside
  4. Effect Of Mangosteen Rind Extract (garcinia Mangostana L.) To Necrosis Alveolar Type Ii Cell Of Lungonpregnant Mice (mus Musculus)exposured By Cigarette Smoke
  5. Histopathological Appearance Of Gastrointestinal Tract Iv Instar Mosquito Larvae Of Aedes Aegypti After Dipping With Active Compounds Of Permot Leaf Extract (passiflora Foetida Linn.) And Its Potential As Bioinsecticide
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  7. The Seminal Plasma Protein Of Cow Sustain Quality Of Beans Goat Spermatozoa After Equilibration In Freezing Process
  8. The Potential Of Garlic (allium Sativum) Infuse To Caecum Of Broilers Histopathology Infected By Escherichia Coli
  9. The Effectiveness Of Giving Haemopoetic Stem Cells In The Pregnant Mice (mus Musculus) Exposed To Plumbum Towards The Decreasing Number Of Necrocis Cells And Expression Of Caspase-3 In Placenta
  10. Protective Effect Of Binahong Leaf Extract [anredera Cordifolia (ten.) Steenis] To Renal Histopathological Of Rat (rattus Norvegicus) Induced With Borax (na2b4o710h2o)
  11. Effect Of Antibacterial Traditional Apple Vinegar And Apple Vinegar Products On The Growth Of Staphylococcus Aureus In Vitro
  12. The Effect Of Mangosteen Peel Extract (garcinia Mangostana L.) To The Number Of Placental Trophoblast Cells In Mice (mus Musculus) Exposure To Cigarette Smoke
  13. Analysis Of Humoral Immune Response (igg) In Rabbit Immunized With Proteins Of Leucocytozoon Caulleryi
  14. Isolation Of Methicillin-resistant Staphylococcus Aureus (mrsa) In Dog Nasal Swab With Oxacillin Screen Agar Confirmation Test
  15. Effect Of Prostaglandin F2α, Pregnant Mare Serum Gonadotropin, And A Combination Of Pregnant Mare Serum Gonadotropin And Human Chorionic Gonadotropin On Pregnancy Rates And Litter Size Of Mice
  16. Through The Provision Of Drinking Water Multiprobio Against Performan Broiler Chicken Production

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