Veterinaria Medika
ISSN 1979-1305
Vol. 7 / No. 1 / Published : 2014-02
Order : 11, and page :67 - 72
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Original Article :
The pattern of antimicrobial susceptibility of salmonella sp isolated from beef
Author :
- A.T. Soelih Estoepangestie.*1
- Freshca Ayu Anggita,*2
- Boedi Setiawan*3
- Dosen Fakultas Kedokteran Hewan
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
The aim of the study was to determine Salmonella sp susceptibility to antibiotic. Samples were obtained from beef then were isolated and identified of Salmonella sp due to it’s characteristic of rod shape, Gram-negative, black coloni on BSA medium, and the ability to produce alkalis - base, gas and H2S in TSIA medium, and Citrat positive. Seven isolates out of 21 samples were identified as Salmonella sp. The Antibiotica susceptibility was than tested by disk diffusion method according to Kirby-Bauer using 10 antibiotic disks as follows: Ampicillin, Ciprofloxacin, Chloramphenicol, Cloxacillin, Erythromycin, Gentamycin, Nalidixic Axid, Oxytetracycline, Penicillin and Tetracycline. The result showed that: 100 % Salmonella sp isolates were susceptible to Gentamycin and Ciprofloxacin; 42,8% were intermediate to Chloramphenicol, 28,5% were intermediate to Nalidixic Axid; and only 14,3% were susceptible to chloramphenicol, Nalidixic Axid, Oxytetracycline and Tetracycline; while it were 100% Resistance to Ampicillin, Cloxacilline, Erythromycin and Penicilline; 85,7% were resistant to Oxytetracycline and Tetracycline; 57,2% were resistant to Nalidixic Axid and 42,8% were resistance to Chloramphenicol.
Keyword :
Salmonella sp., Beef shank, Susceptibility test,
References :
Dewan Standardisasi Nasional,(2009) SNI Batas Cemaran Mikroba Dalam Bahan Pangan Asal Ternak (SNI 7388:2009) Jakarta : Dewan Standardisasi Nasional
Prawesthirini, S. Dan H.P. Siswanto., Estoepangestie, A.T.S., Effendi, M.H., Harijani, N., Budiarto,(2011) Analisa Kualitas Susu, Daging, dan Telur Surabaya : Fakultas Kedokteran Hewan Universitas Airlangga
Setiowati, E.W. dan E. Mardiastuty,(2009) Tinjauan Bahan Pangan Asal Hewan Yang Asuh Berdasarkan Aspek Mikrobilogi di DKI Jakarta Jakarta : Prosiding PPI Standardisasi
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