UNIVERSITAS AIRLANGGA



Detail Article

Majalah Ilmu Faal Indonesia

ISSN 0215-1995

Vol. 6 / No. 3 / Published : 2007-10

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Original Article :

Pola mutan gen glukosa 6 fosfat dehidrogenase di surabaya

Author :

  1. Suhartati -*1
  1. -

Abstract :

Glucose 6 phosphate dehydrogenase (G6PD) deficiency was studied in open Surabaya region. Seventy-six blood samples were collected from Surabaya The samples were screened using Randox Kit to determine the activity of G6PD, taking a cut-off value of less than 60% as indicating G6PD deficiency. Thirty one of cases tested positive with the Randox Kit test, 26 of which tested positive with MPTP. In this study, genomic DNA from blood samples were extracted using simple rapid genomic DNA method. The extracted DNA was then used to amplify exon 5, 6, 9, 11, and 12 of the G6PD genes to detect mutants using Multiplex PCR with Multiple Tandem forward Primers and a common reverse primer (MPTP) technique according to Shirakawa. Seven different G6PD mutants were found in Surabaya: 4 cases of Vanua Lava, a single G6PD Viangchan cases, 5 cases of G6PD Chatham, 2 cases of G6PD Union, 7 cases of Canton , 6 cases of Kaiping and 2 cases of Silent mutation. Double mutants were also found, all involving silent mutations: Canton + Silent, Vanua Lava + Silent, and Viangchan + Silent, one case each. Nine cases of G6PD deficiency were found in women with a history of miscarriages : 3 cases of Canton, 3 cases of Kaiping, one single case of Vanua Lava and 2 tested negative with MPTP. G6PD deficiency is usually asymptomatic. Symptoms will only appear if those persons ingest oxidants such as certain drugs or foodstuffs. The disorders manifest itself as hemolytic anemia, repeated hepatitis, repeated miscarriages etc. Since G6PD deficiency is more or less prevalent in Indonesia, an early diagnosis would be of advantage, so that people with G6PD deficiency could avoid ingesting oxidants

Keyword :

G6PD deficiency, MPTP, history of miscarriages, oxidants,


References :





Archive Article

Cover Media Content

Volume : 6 / No. : 3 / Pub. : 2007-06
  1. Pola Mutan Gen Glukosa 6 Fosfat Dehidrogenase Di Surabaya
  2. Perbedaan Latihan Fisik Jangka Pendek Dan Jangka Panjang Terhadap Glukosa Darah Pada Penderita Diabetes Mellitus
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  4. Pemberian Tambahan Kalsium Pada Masa Pertumbuhan Terhadap Tebal Tulang Kortikal Dan Trabekular
  5. Physiological Aspects Of Testosterone Administration In Andropause
  6. Perbedaan Persepsi Pengecap Rasa Asin Antara Usia Subur Dan Usia Lanjut
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