OVOZOA
ISSN 2302-6464
Vol. 3 / No. 1 / Published : 2014-04
Order : 13, and page :215 - 219
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Original Article :
Effect of equilibration time extender to post thawing motility and viability of merino sheep’s sperms in egg yolk skim diluents
Author :
- Fajarillah Nurul Hayati*1
- Suwarno*2
- Trilas Sardjito*3
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
The aims of this research was to determine effect of equilibration time extender to post thawing motility and viability of Merino Sheep’s sperms in egg yolk skim diluents. This research used Merino Sheep’s fresh semen that collected in artificial vagina and divided to four treatment. The first treatment (P1) was semen equilibration 1 hour. The second treatment (P2) was semen equilibration 1,5 hour. The Third treatment (P3) was semen equilibration 2 hour. The fourth treatment (P4) was semen equilibration 2,5 hour. Experiment design used was randomized complete draft. The result was analysed used ANOVA (Analysis of Variant) and continued with different test (the smallest real BNT 5%). The post thawing motility’s result was (P1)18,00 ± 2,74, (P2) 25,00 ± 3,54, (P3) 34,00 ± 2,24 and (P4) 29,00 ± 4,18. The post thawing viability’s result was (P1) 31,00 ± 3,03, (P2) 39,00 ± 4,39, (P3) 51,00 ± 5,03, (P4) 44,00 ± 4,95, showed that the inherent motility and viability different treatment groups significantly with the third treatment (P3) was semen equilibration 2 hour.
Keyword :
equilibration, motility, viability, egg yolk skim,
References :
Almahdy, H., M. W. Tess, E. El-Tawil, E. Shehata, and H. Mansour. ,(2000) Evaluation of Egyptian Sheep Produc-tion System: I. Breed Crosses and Management Systems. USA : J. Anim. Sci. 78:283-287.
Herdis, B., Purwantara, I. Supriatna dan I.G Putu. ,(1999) Integritas Spermatozoa Ker-bau Lumpur (Bubalis bubalis) pada Berbagai Metode Pembekuan Semen. Bogor : DJurnal Ilmu Ternak dan Veteriner Nomor 1 (4). 1999. 7-12.
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