OVOZOA
ISSN 2302-6464
Vol. 1 / No. 1 / Published : 2012-10
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Original Article :
Motility and viability ram’s spermatozoa on various silver ion (ag+) concentration in egg yolk citrate coconut water assortment extender
Author :
- Hardijanto*1
- Isma Ari Vidyana*2
- Anwar Ma’ruf*3
- Departemen Reproduksi Fakultas Kedokteran Hewan Unair
- Mahasiswa Fakultas Kedokteran Hewan Unair
- Departemen Kedokteran Dasar Fakultas Kedokteran Hewan Unair
Abstract :
ABSTRACT The aims of this research was to find out the influence of various silver ion concentration in egg yolk citrate coconut water assortment extender in motility and viability of ram’s spermatozoa. Semen was divided into three groups of extenders. Control Group (P0) using Egg Yolk Citrate Coconut Water-Antibiotic, group 1 (P1) using Egg Yolk Citrate Coconut Water-Silver Ion 1,5 ppm, group 2 (P2) using Egg Yolk Citrate Coconut Water-Silver Ion 0,75 ppm, group 3 (P3) using Egg Yolk Citrate Coconut Water-Silver Ion 0,375 ppm and group 4 (P4) using Egg Yolk Citrate Coconut Water-Silver Ion 0,1875 ppm. Its were monitored every day at the same time in six days after storage at 5°C. The results showed that the degradation of motility percentage in P4 was relative stabilize in comparation with P0, P1, P2 and P3. Motility of P0, P1, P2, P3 and P4 was not significantly different (p>0,05). Viability of P1, P2, P3 and P4 was not significantly different (P>0,05) until fourth day but P0 was significantly different (p>0,05) in fifth day. The conclusion of this experiment is Various Silver Ion in Egg Yolk Citrate Coconut Water assortment extender have the same influence in motility of ram’s spermatozoa and have the same influence in viability of ram’s spermatozoa until fourth day but different influence on fifth and sixth day.
Keyword :
Silver Ion, Egg Yolk, Citrate, Ram’s Spermatozoa,
References :
Acel, D. ,(1994) Antimicrobial Activity and Action of Silver. Prog Med Chem. 31 :353 Baltimore : Prog Med Chem
Do Comm CNMO, E.,(2003) Viral Pathogens and Severe Acute Respiratory Syndrome: Oligodynamic Ag1 for Direct Immune Intervention. Journal of Nutritional & Environmental Medicine 13(2), 109–118 : Journal of Nutritional & Environmental Medicine
Waley, J. and Sonns. ,(2000) Mechanistic Study of the Antimicrobial Effect of Silver Ions. Inc. J. Biomed Mater Res. 52:662-668 : J. Biomed Mater Res.
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