OVOZOA
ISSN 2302-6464
Vol. 6 / No. 1 / Published : 2017-04
Order : 11, and page :50 - 54
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Original Article :
Pengaruh penambahan plasma seminalis sapi simmental terhadap motilitas dan viabilitas spermatozoa domba ekor gemuk setelah ekuilibrasi pada proses pembekuan
Author :
- Pamela Dewi Asia*1
- Prof. Dr. Suherni Susilowati, drh., M.Kes.*2
- Retno Bijanti,drh., M.S.*3
- Prof. Dr. Rr. Sri Pantja M., drh., M.Si*4
- Dr. Tatik Hernawati, drh., M.Si.*5
- Dr. Tjuk Imam Restiadi, drh., M.Si.*6
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
The aims of this research was to determine effect of addition Seminal Plasm of simmental bull to motility and viability of ram’s spermatozoa after equilibration on freezing process. This research consists of three treatments. Control treatment (PO) were diluter + ram’s spermatozoa (without seminal plasma). Treatment I (PI) were diluter + ram’s spermatozoa + bull’s seminal plasma (1:1), treatment II (P2) were diluter + ram’s spermatozoa + bull’s seminal plasma (1:2). All of treatments was observed of motility and viability of ram’s spermatozoa after equilibration. The motility’s result was (P0) 55,8 ± 3,7, (P1) 64,1 ± 3,7, and (P2) 70,0 ± 3,1. The viability’s result was (P0) 64,4 ± 3,2, (P1) 71,5 ± 4,1, and (P2) 83,9 ± 3,4. The results showed that highest rate in maintaining motility and viability of ram’s spermatozoa among all of treatments was P2. It can be concluded that addition of seminal plasm of simmental bull with the ratio 1: 2 is effective in maintaining motility and viability of ram’s spermatozoa after equilibration.
Keyword :
Seminal plasma, Simmental bull, ram’s spermatozoa, motility, viability.,
References :
Amin, M. R., Mozes R. Toelihere, Tuty L. Yusuf, and Polmer Situmorang.,(1999) Effects of bovine seminal plasma on frozen semen quality of swamp buffaloes (Bubalus bubalis). 4(3): 143 - 147. : Jurnal Ilmu Ternak dan Veteriner
Guthrie, HD and Welch, GR.,(2012) Effects of Reactive Oxygen Species On Sperm Function. 78(8):1700. : PubMed Theriogenology.
Hayati A, Mangkoewidjojo S, Hinting A, Moeljopawiro S.,(2006) Hubungan kadar MDA sperma dengan integritas membran sperma tikus (Rattus nervegicus) setelah pemaparan 2-Mothoxyethanol. 11:151-154. : Berk Penel Hayati.
Herdis., M. R. Toelihere, I. Supriatna, B. Purwantara dan RTS. Adikara.,(2005) Optimalisasi Kualitas Semen Cair Domba Garut (Ovis aries) melalui Penambahan Maltosa ke dalam Pengencer Semen Tris Kuning Telur. 21: 2. : Media Kedokteran Hewan.
Sukmawati, E., R.I. Arifiantini, dan B. Purwantara.,(2014) Daya Tahan Spermatozoa terhadap Proses Pembekuan pada Berbagai Jenis Sapi Pejantan Unggul. 19(3): 168-175.
Archive Article
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