OVOZOA
ISSN 2302-6464
Vol. 7 / No. 1 / Published : 2018-04
Order : 12, and page :58 - 63
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Original Article :
Pengaruh sari kulit dan buah semangka merah (citrullus lanatus) sebagai bahan pengencer terhadap motilitas dan viabilitas spermatozoa domba
Author :
- Faisal Andrianto*1
- Prof. Dr. Suherni Susilowati, drh., M.Kes.*2
- Dr. Kusnoto, drh., M.Si.*3
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
Aim of this research was to study the effect of peel and flesh of watermelon as diluter on motility and viability of ram spermatozoa. There were three treatments: 1) KTS= egg yolk citrate; 2) BSS= watermelon flesh extract citrate; 3) KSS= watermelon peel extract citrate. Each treatments were stored at 5oC and observed for five level of storage time, which were day 1, day 2, day 3, day 4, and day 5. Data were analysed using completely randomised design with factorial pattern 3x5 followed by duncan multiple range test if there were significant differences (P<0.05). Result showed there was interaction between treatments and storage times which were significantly different (P<0,05) towards motility and viability of spermatozoa. The highest percentage of motility and viability were obtained from BSS for 1 day of storage which were 76.25±4.787% and 82.75±3.304%. The lowest percentage of motility and viability were obtained from KSS for 5 days of storage which were 23.75±2.062% and 31.75±2.363%. From the overall storage time, the average spermatozoa motility and viability’s percentage of KTS showed the best result which were 58.00±13.183% and 66.00±10.959%. Conclusion of this research was the extract of watermelon peel and flesh can be used as a diluter only for four days of storage time. Therefore addition of other substance which can protect spermatozoa from cold shock was needed to extend storage time more than four days.
Keyword :
ram semen, motility, viability, watermelon (Citrullus lanatus),
References :
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