Jurnal Rekonstruksi & Estetik
ISSN 2301-7937
Vol. 2 / No. 1 / Published : 2013-01
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Original Article :
Pengaruh pemberian topikal low molecular weight hyaluronate pada epitelialisasi luka superfisial tikus putih yang dirawat dengan membran amnion freeze-dried
Author :
- Hartono Kartawijaya*1
- M. Sjaifuddin Noer*2
- mahasiswa
- dosen
Abstract :
Backgrounds Partial/superficial thickness wounds frequently seen in clinical practice, whereas in major burns lead to the devastating effects. Various wound dressing had been proposed with various success, but fresh amnion (FA) as a biological dressing remained superior by its cost, availability, easy to apply and growth-factors contained with enhancement of wound healing properties. Recent concern about the risk of disease transmission clearly abrupt clinical use of FA worldwide. The freeze-drying method for amnion preservation was mostly acceptable and became standard method in Dr.Soetomo Biomaterial Centre and Tissue Bank Surabaya with sterile condition granted by Gamma irradiation. Unfortunately, several research had shown no superiority of freeze-dried amnion (FD) compared with other modalities. These due to significant decreased amount of growth factors. Recently revealed that FA contain of High Molecular Weight Hyaluronate which contibuted to wound healing by its natural degradation into Low Molecular Weight. Addition of Low Molecular Weight Hyaluronate (LMWHA) were expected to enhance wound healing by FD. Methods Twenty four Wistar rats (Rattus norvegicus) were wounded superficially on the back torso in 3 locations of each. One was covered with fresh amnion (FA Group), one covered with freeze-dried amnion (FD Group), the other covered with freeze-dried amnion + LMWHA 1% (HA Group). Samples collected randomly at day 1,3,5 and 7 with 6 rats sacrified at once. Histological changes were observed for the amount of epithelial layer, epithelial thickness and maturation. Data were distributive analyzed by Kolmogorov-Smirnov and colleration analyzed by Anova and Kruskal-Wallis test. Results No epithelization demonstrated at day 1 in all groups. HA group had epithelization rate more inferior than FA group in day 3, but showed superiority in day 5 and 7 (p<0,05). HA group had epithelization rate more superior than FD group in day 3,5 and 7 (p<0,05). FA group had epithelizaion rate more superior than FD group in day 3 and 7 (p<0,05) but not in day 5 (p>0,05). FA group showed better in epithelial maturation compared in two other groups but there’s not significant (p>0,05)
Keyword :
LMWHA, Fresh amnion, Freeze-dried amnion, growth factors, enhance wound healing,
References :
Heimbach DM & Bozarth J,(2006) Acute Burn Nutrition In : Burn Surgery : Reconstruction and Rehabilitation Philadelphia : Elsevier
Jenkins RH, Williams JD & Steadman R,(2005) Fibroblasts Transformed to a Wound Healing Phenotype Accumulate a Hyaluronan-Rich Extracellular Matrix Through Reduced Degradation Philadelphia : Elsevier
Archive Article
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 Volume : 2 / No. : 1 / Pub. : 2013-01 |
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