UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 10 / No. 2 / Published : 2017-07

Order : 8, and page :183 - 190

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Original Article :

Isolation of keratinolytic bacteria from chicken feather waste

Author :

  1. Risa Septiana*1
  2. M. Anam Al Arif*2
  3. E. Bimo Aksono*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This study was aimed to isolate keratinolytic bacteria of chicken featherwaste and testkeratinolytic activities qualitatively. Samples obtained from chicken feather waste whichwere buried in the soil in order to degraded naturally. Bacterial isolation that could befound weresix keratinolytic bacterial. All of six isolates were Gram-positive and coccusmorphology. The keratinolytic activity test (ability to hydrolyze keratin) wereconductedby inoculationof bacteria in paper disc then were placed on FMA (Feather Meal Agar)and were incubated for 24 hours at 37ºC, stained with congo red 0.1% and incubatedfor 30 minutes then rinsed with NaCl 1%, the result was observedthe halo or a clearzone and measured in diameter. Three isolates that had the greatest keratinolytic activitycharacterized by the widest diameter clear zone isthe isolate A1.3, A1.1, B1.2with anaverage twice that of bacterial colony (paper disc). Paper disc diameter is 6 mm, isolatesA1.3 diameter is 13,85 mm,isolates A1.1 diameteris 13,50mm, isolates B1.2 diameter is12,45 mm.

Keyword :

keratinolytic bacteria, chicken feathers, isolation,


References :

Adiati, U., Puastuti, W., Mathius, I-W.,(2004) Peluang Pemanfaatan Tepung Bulu Ayam sebagai Bahan Pakan Ternak Ruminansia 14 (1):39-44 : Wartazoa

Agrahari, S., Wadhwa, N.,(2010) Degradation of Chicken Feather a Poultry Waste Product by Keratinolytic Bacteria Isolated from Dumping Site at Ghazipur Poultry Processing Plant 9(5): 482-489 : International Journal of Poultry Science

Brandelli, A., Daroit, D. J. and Riffel, A.,(2010) Biochemical Features of Microbial Keratinases and Their Production and Applications 85:1735-1750 : Appl Microbiol and Biotechnol





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Volume : 10 / No. : 2 / Pub. : 2017-07
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  2. Effectiveness Of Red Fruit (pandanus Conoideus Linn) Oil Administration On Infertile Mice (mus Musculus) Serum Luteinizing Hormone And Corpus Luteum Number
  3. The Influence Of Beta Carotene To Live Fetus Percentage In Mice (mus Musculus) That Exposured By Cigarette Smoke
  4. Immune Response Of Mice (mus Musculus) By Vaccinated Brucella Abortus Strain Rb 51 And Infected With Brucella Suis Toward Spleen Histopathology
  5. Activity Of Alkaloid Of Jarong Leaves (achyranthes Aspera Linn ) On The Death Of Cancer Cells In The Breasts Of Mice (mus Musculus)
  6. Isolation And Identification Of Edwardsiella Tarda Bacteria In Clarias Gariepinus Place Region In Jombang
  7. Effect Of Rosella Flower Extract (hibiscus Sabdariffa Linn.) On Kidney Histopathologic Of Male Mice (mus Musculus) Exposed Lead Acetate
  8. Isolation Of Keratinolytic Bacteria From Chicken Feather Waste
  9. The Pathology Of African Catfish (clarias Gariepinus) Kidney Infected By Edwardsiella Tarda
  10. Featuring Liver Pathology Of Clarias Gariepinus That Were Infected By Edwardsiella Tarda
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  13. The Effects Of Vitamin C Toward Histopathology Of Stomach Of Mice Exposed To Endosulfan
  14. The Effect Of Alkaloid Achyranthes Aspera Linn On Cytochrom C And Apaf 1 Expresion On Mice Breast Cancer Was Induction By Benzopyrine
  15. Imunomodulator Potention Of Alkaloid Sambiloto (andrographis Paniculata L) To Ifnγ And Cd4+ Response After Infected By Salmonella Typhimurium On The Rat
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