Veterinaria Medika
ISSN 1979-1305
Vol. 10 / No. 2 / Published : 2017-07
Order : 8, and page :183 - 190
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Original Article :
Isolation of keratinolytic bacteria from chicken feather waste
Author :
- Risa Septiana*1
- M. Anam Al Arif*2
- E. Bimo Aksono*3
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
This study was aimed to isolate keratinolytic bacteria of chicken featherwaste and testkeratinolytic activities qualitatively. Samples obtained from chicken feather waste whichwere buried in the soil in order to degraded naturally. Bacterial isolation that could befound weresix keratinolytic bacterial. All of six isolates were Gram-positive and coccusmorphology. The keratinolytic activity test (ability to hydrolyze keratin) wereconductedby inoculationof bacteria in paper disc then were placed on FMA (Feather Meal Agar)and were incubated for 24 hours at 37ºC, stained with congo red 0.1% and incubatedfor 30 minutes then rinsed with NaCl 1%, the result was observedthe halo or a clearzone and measured in diameter. Three isolates that had the greatest keratinolytic activitycharacterized by the widest diameter clear zone isthe isolate A1.3, A1.1, B1.2with anaverage twice that of bacterial colony (paper disc). Paper disc diameter is 6 mm, isolatesA1.3 diameter is 13,85 mm,isolates A1.1 diameteris 13,50mm, isolates B1.2 diameter is12,45 mm.
Keyword :
keratinolytic bacteria, chicken feathers, isolation,
References :
Adiati, U., Puastuti, W., Mathius, I-W.,(2004) Peluang Pemanfaatan Tepung Bulu Ayam sebagai Bahan Pakan Ternak Ruminansia 14 (1):39-44 : Wartazoa
Agrahari, S., Wadhwa, N.,(2010) Degradation of Chicken Feather a Poultry Waste Product by Keratinolytic Bacteria Isolated from Dumping Site at Ghazipur Poultry Processing Plant 9(5): 482-489 : International Journal of Poultry Science
Brandelli, A., Daroit, D. J. and Riffel, A.,(2010) Biochemical Features of Microbial Keratinases and Their Production and Applications 85:1735-1750 : Appl Microbiol and Biotechnol
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