OVOZOA
ISSN 2302-6464
Vol. 2 / No. 2 / Published : 2013-10
Order : 7, and page :49 - 56
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Original Article :
Fertilization rate of merino sheep frozen semen in diluent egg yolk tris and egg yolk skim to in vitro fertilization
Author :
- Erin Nuraisa*1
- Pudji Srianto*2
- Widjiati*3
- Mahasiswa Fakultas Kedokteran Hewan Unair
- Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Unair
- Departemen Anatomi Veteriner Fakultas Kedokteran Hewan Unair
Abstract :
The aim of this study was to evaluate fertilization rate of Merino sheep frozen semen that processed by using two kind of diluter egg yolk tris (P1) and egg yolk tris (P2). Merino sheep semen was taken by using artificial vagina than divided by two treatments. One part was diluted with egg yolk tris and the other with egg yolk skim. Fertilization rate was evaluated by In Vitro Fertilization (IVF) methode. Data was analized by using t-test. The result showed that there was no significance difference between two treatments (P>0,05). This study conclude that either egg yolk tris or egg yolk skim can be used as a diluter for frozen semen processing of Merino sheep. Both of them can produce almost equal fertilization rate.
Keyword :
Merino sheep, egg yolk tris, fertilization in vitro, fertilization rate,
References :
Crozet N, M. Ahmed-Ali and M.P. Dubos. ,(1995) Developmental competence of goat oocytes from follicles of different size categories following maturation, fertilization and culture in vitro. USA : Reprod. Fertil. 103: 293-298.
Widjiati, B. Prajogo E.W., T. Sardjito dan N. Harijani. ,(1998) Pengaruh Gandarussa vulgaris Ness terhadap Kultur Embrio Mencit. Surabaya : Media Kedokteran Hewan. 14:200-204.
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