UNIVERSITAS AIRLANGGA



Detail Article

Veterinaria Medika

ISSN 1979-1305

Vol. 3 / No. 3 / Published : 2010-11

Order : 15, and page :231 - 238

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Original Article :

Motility and live percentage test in ram’s spermatozoa in isotonic commercial liquid and egg yolk assortment extender

Author :

  1. Cindy Ichmy*1
  2. Hardijanto*2
  3. Kusriningrum Rochiman S*3
  1. PPDH Fakultas Kedokteran Hewan Unair
  2. Fakultas Kedokteran Hewan Universitas Airlangga
  3. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The successful program of artificial insemination in ram is determined by the quality of sperm that are used. Therefore, it is important to maintain the quality of sperm during and after storage in low temperature. The aims of this study was to find out the influence of Isotonis Commercial Liquid (ICL) and Egg Yolk assortment extender in motility and live percentages of ram’s spermatozoa. Sperm was collected once every three days and eight times using artificial vagina from the same ram. Semen was divided into three groups of extenders. Control Group (P0) using Egg Yolk-Citrate (1:4), group 1 (P1) using Egg Yolk-Isotonic Commercial Liquid (1:4) and group 2 (P2) using Egg Yolk- Isotonis Commercial Liquid-Citrate (1:2:2). Its were monitored every day at the same time in seven days after storage at 5°C. The results showed that the degradation of motility percentage in P0 and P2 was relative stabilize in comparation with P1 experiencing of significant degradation at the third day and none at the fourth day. Live percentages of P0 and P2 was significantly different (P<0,05), P1 experiencing of significant degradation at the third day of motility percentage and death in fifth day. The conclusion of this experiment is Isotonic Commercial Liquid (ICL) and Egg Yolk assortment extender can be influence motility and live percentages of ram’s spermatozoa.

Keyword :

Isotonic Commercial Liquid, Egg Yolk, Citrate, Ram’s Spermatozoa, .,


References :

Damayanti, Y.,(1991) Pengaruh Kadar Fruktosa dalam Pengencer Air Kelapa Muda, Air Siwalan dan Kombinasinya dengan Kuning Telur terhadap Kualitas Air Mani Ayam Buras Surabaya : Fakultas Kedokteran Hewan Universitas Airlangga

Hardijanto, S. Susilowati, T. Sardjito, T. Hernawati, dan T.W. Suprayogi.,(2008) Penuntun Pratikum Fisiologi dan Teknologi Reproduksi (Inseminasi Buatan) Surabaya : Fakultas Kedokteran Hewan. Airlangga University Press

Herdis, M. R. Toelihere, I. Supriatna, B. Purwantara dan R.T.S. Adikara,(2005) Optimalisasi Kualitas Semen Cair Domba Garut (Ovis aries) melalui Penambahan Maltosa ke dalam Pengencer Semen Tris Kuning Telur Bogor : Media Kedokteran Hewan





Archive Article

Cover Media Content

Volume : 3 / No. : 3 / Pub. : 2010-11
  1. Garlic As An Alternative Disinfectant To The Escherichia Coli
  2. Centrifugation Of Goat Semen At Freezing Process With Some Concentrate Of Egg Yolk Diluent To The Quality Of Sperm
  3. Influence Of The Avian Influenza Virus Subtype H5n1 Infection Toward Sperm Motility And Life In Long Tail Monkey (macaca Fascicularis)
  4. Vfa Concentration And Acetate, Propionate, Butyrate Molar Proporsions Rumen Of Ongole Grade Cattle With Ammoniation Rice Straw, Soybean Straw And Rice Straw
  5. The Activity Of Achyranthes Aspera Linn Alkaloid To Cause Aopoptotic And Dna Fragmentation On Breast Cancer
  6. The Antimitogenic Effect Of Alkaloid Achyranthes Aspera Linn On Apoptotic Induction In Cell Infection By Mycobacterium Tuberculosis
  7. Stages Of Vaccination And The Type Of Vaccine Used To Prevent Infectious Disease In Chickens
  8. Effect Of Complex Of Insulin Like Growth Factor-1 And Insulin Like Growth Factor Binding Protein-3 On The Increase Number Of Ovarian Follicle In Female Mice (mus Musculus)
  9. Spleen Weight And White Pulp Diameter Picture In Broiler Chickens Exposed To Cronic Heat Stress
  10. Identification Of Kit-ligand From In Vitro Maturation Of Bovine Oocytes With Electrophoresis Method
  11. Cellulolityc Bacteria For Increasing Quality Of Complete Feed From Waste Product
  12. Identification Of Cyclin-dependent Kinase (cdk1) That Express On Bovine Cumulus Oocyte Complex After A Model Oocyte Maturation Experimence
  13. Utilization Of Bakteriocin In The Handling Of Sub Clinical Mastitis And The Effect On Milk Quality On The Number Of Bacteria
  14. Expression Of Adhf-36kda Protein Towards Osmolarity And Ph In Vitro Environmental Of Salmonella Typhi (as Study To Adhesin Molecule-based Candidate Vaccine For Typhoid Fever)
  15. Motility And Live Percentage Test In Ram’s Spermatozoa In Isotonic Commercial Liquid And Egg Yolk Assortment Extender
  16. Identification Of The Gene For Staphylococcus Aureus Toxic Shock Syndrome Toxin-1 (tsst-1) From Bovine Mastitis Milk

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