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Jurnal Ilmiah Biologi FST

ISSN 2303-3428

Vol. 3 / No. 1 / Published : 2015-01

TOC : 56, and page :56 - 66

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Original Article :

Pengaruh intensitas cahaya terhadap biomassa dan kadar saponin kalus ginseng jawa (talinum paniculatum gaertn.) pada berbagai waktu kultur

Author :

  1. Afifatul Alwiyah,*1
  2. Y. Sri Wulan Manuhara*2
  3. Edy Setiti Wida Utami*3
  1. Mahasiswa Fakultas Sains dan Teknologi
  2. Dosen Fakultas Sains dan Teknologi
  3. Dosen Fakultas Sains dan Teknologi

Abstract :

The aims of this research was to study the effects of light intensity on callus biomass and saponin content of ginseng jawa (Talinum paniculatum Gaertn.). Callus induced from leaf explants on solid Murashige and Skoog (MS) medium supplemented by 2 mg/L of 2,4-D and 1 mg/L of kinetin. Callus induced by treatment of combination between light intensities 1000, 2000, 4000, 6000 lux, also dark condition and culture period 4, 6, and 8 weeks. Each treatment of combination was repeated 6 times. Retrieval of data were callus biomass (fresh and dry weight) and saponin content. The data of biomass were analyzed by using manova test (significance of 5%). Saponin content was analyzed semi-quantity using Thin Layer Chromatography (TLC). The result showed that the highest average of dry weight (as parameter) obtained at treatment combination 4000 lux light intensity and 8 weeks culture period was 0,0699 gram. Meanwhile the highest saponin content obtained at 4000 lux light intensity and 6 weeks culture period was 58,5 mm2/0,01 gram dry weight of callus and color intensity +4. With the results that light intensities influence on callus biomass and saponin content of ginseng jawa at different culture period.Keywords: biomass, callus, culture period, light intensity, saponin content, Talinum paniculatum Gaertn

Keyword :

biomass,


References :

  1. Abe, H.,, (1980). Pharmacological actions of saikosaponins isolated from Bupleurum falcatum.. Planta Medica 40: 366-372 : Effects of saikosaponin on liver function, Planta Medica 40: 366-372
  2. Blando, F., A. P. Scardino, L. De Bellis, I. Nicoletti, and G. Giovinazzo,, (2005). Characterization of in vitro anthocyanin-producing sour cherry (Prunus cerasus L.). Food Research Internat 38: 937-942 : callus culture,
  3. Khristiyana, L., E. Anggarwulan., dan Marsusi., (2005). Pertumbuhan, kadar saponin, dan nitrogen jaringan tanaman daun sendok (Plantago major L.) pada pemberian asam giberelat (GA3)),. Jurnal Biofarmasi 3 (1): 1693-2242 : Jurnal Biofarmasi 3 (1): 1693-2242


   


Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2015-01
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