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Jurnal Ilmiah Biologi FST

ISSN 2303-3428

Vol. 3 / No. 1 / Published : 2015-01

TOC : 47, and page :47 - 55

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Original Article :

Pengaruh konsentrasi sukrosa terhadap biomassa dan kadar saponin kalus ginseng jawa(talinum paniculatum gaertn.) pada berbagai waktu kultur

Author :

  1. Deshinta Elsa Lina,*1
  2. Y. Sri Wulan Manuhara*2
  3. Hery Purnobasuki,*3
  1. Mahasiswa Fakultas Sains dan Teknologi
  2. Dosen Fakultas Sains dan Teknologi
  3. Dosen Fakultas Sains dan Teknologi

Abstract :

The aim of this research was to determine the effect of sucrose concentration on callus biomass and saponin levels of ginseng java (Talinum paniculatum Gaertn.) in various culture of predetermined time. The research was conducted by inducing the callus on MS medium given growth regulator 2,4-D 2 mg/L kinetin and 1 mg/L and sucrose levels variation different at 15 g /L, 30 g /L (control) , 60 g /L, and 90 g /L. Callus was grown in a culture that is different in each sucrose treatment. Given culture time was 4 weeks, 6 weeks and 8 weeks. The parameters that were measured include fresh weight, dry weight and the levels of saponins. Levels of saponins were obtained from the calculation of the area of the stain on silica gel GF254 plates. The results of research were tested with MANOVA (Multivariate Analysis of Variance). From the results of present study are known that the effect of sucrose concentration on callus biomass and ginseng saponin content of Java (Talinum paniculatum Gaertn.) at various times of culture. The concentration of sucrose is right for the getting the best biomass is the giving sucrose 60 g/L at the culture time of 6 weeks for fresh weight and 60 g/L at 8 weeks for dry weight. While the best saponin levels produced at sucrose concentration of 90 g/L at 8 weeks of culture.Keywords: Callus, culture time, saponin, sucrose, Talinum paniculatum Gaertn.

Keyword :

Callus, , culture time,


References :

  1. Hariana, A,., (2008). Tumbuhan Obat dan Khasiatnya Seri 3.. seri 3 : Penebar Swadaya: Jakarta.
  2. Jo, J. S, Han, Y, N., Oh, H. I., Park, H., Sung, H. S. and Park, J. I,., (1995). Korean ginseng has a characteristic shape. In understanding of Korean ginseng,. Hanrimwon Publishing Co, Seoul, Korean. : Hanrimwon Publishing Co, Seoul, Korean.
  3. Ikhtimami, A,, (2012). Pengaruh periode subkultur terhadap kadar saponin akar rambut tanaman ginseng jawa (Talinum paniculatum Gaertn.),. Skripsi, : Skripsi, Universitas Airlangga: Surabaya


   


Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2015-01
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  7. Pengaruh intensitas cahaya terhadap biomassa dan kadar saponin kalus ginseng jawa (talinum paniculatum gaertn.) pada berbagai waktu kultur
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