Berkala Ilmiah Kimia Farmasi
ISSN 2302-8270
Vol. 4 / No. 2 / Published : 2015-11
Order : 3, and page :12 - 18
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Original Article :
Isolasi dan penapisan fibrinolitik jamur tanah hutan mangrove wonorejo surabaya
Author :
- DWI MUISRISTANTO*1
- ACHMAD TOTO POERNOMO*2
- SUGIJANTO*3
- Mahasiswa Fakultas Farmasi
- Dosen Fakultas Farmasi
- Dosen Fakultas Farmasi
Abstract :
Research conducted on twenty soil samples originating from Mangrove Forest Wonorejo, Surabaya taken from ten locations. Sample was diluted to 10-3, cultured on media Potatoes Dextrose Agar (PDA) and tested the proteolytic activity on the media Skim Milk Agar (SMA) 2%. Thirteen of twenty samples were tested gave positive results, it is shown by a clear zone around the colonies of fungus growing. Fungus that gives positive results cultured on PDA slant as stocks of proteolytic fungi isolate. The fungus that gives proteolytic activity then determination fibrinolytic activity on fibrin media plate made of fibrin 0.3% and 1.7% agarose and Methylene Blue 400 µL. Nine samples gave positive fibrinolytic activity. Fibrinolytic enzyme activity index was measured by calculating diameter of the clear zone divided by diameter of the colony. Furthermore, fungal isolates that shown fibrinolytic activity chosen to characterize. Characterization of macroscopic fungi by looking directly form of fungal colonies reverse side method. Characterization of microscopic fungus seen in magnification 400-1000x and identified with references Pictorial Atlas of Soil and Seed Fungi (Watanabe, 2002). Pengenalan Kapang Tropik Umum (Gandjar et al., 1999), Illustrated Genera of Imperfect Fungi (Barnett and Hunter, 1998). There are two genera of fungi, Aspergillus and Penicillium, from Mangrove Eco Tourism Wonorejo, Surabaya, which is capable of producing fibrinolytic enzyme
Keyword :
Screening, Fibrinolytic, Aspergillus, Penicillium , Mangrove soil,
References :
Ashipala, O. K., He, Q,(2007) Optimization of fibrinolytic enzyme production by Bacillus subtilis DC 02 in aqueous two-phase system (poly-ethylene glycol 4000 and sodium sulfate). 99, pp. 4112-4119 : Biosource Technology
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