Detail Article


ISSN 1412-1433

Vol. 13 / No. 1 / Published : 2011-01

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Original Article :

Compartion study between animal model sopk and normally clycle is mmp-9, timp-1 enzym activities and collagen expression (an experiment study in rat)

Author :

  1. Budi Santoso*1
  2. R. Prajitno Prabowo*2
  3. Soetjipto*3
  4. Widjiati*4
  1. Departemen Obstetri dan Ginekologi Fakultas kedokteran, Universitas Airlangga, Surabaya
  2. Departemen Obstetri dan Ginekologi Fakultas kedokteran, Universitas Airlangga, Surabaya
  3. Departemen Biokimia Kedokteran, Fakultas Kedokteran Universitas Airlangga, Surabaya
  4. Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya

Abstract :

This study was aimed � � � � � � � � � � � � � � � � � to explain pathogenesis of occurring ovarian cyst and ovulation disturbance in PCOS case. This study is an experimental study using Rattus novergicus as PCOS model, compared to control study in every estrus cycle phase of Rattus novergicus, ie, proestrous, estrous, metestrous and disestrous phase. Each study group and control group consisted of 5 rats so that there were a total of 25 Rattus novergicus. PCOS model group was injected with testosterone propionate with dose 1mg/ 100 BW, whereas control group with propylene glycol injection. The examination of MMP-9, TIMP-1 activity by ELISA activity and Collagen-4 used immunohistochemistry staining. The statistic analysis used ANOVA test. The average of MMP-9 activity of PCOS model (P): 235.17 (SD: 5.47), Proestrous Group (PG): 257.40 (SD: 0.25), Estrous Group (EG): 262.80 (SD: 2.34), Metestrous Group (MG):254.43 (SD: 6.21), Diestrous Group (DG):240.69 (SD: 4.16). With ANOVA test, PCOS model only indicated a significant difference with Estrus Group: (p = 0.001). The average of TIMP-1 activity of P: 57.15(SD: 2.46), PG: 51.63(SD: 3.81), EG: 16.30 (SD: 2.33), MG: 12.84 (SD: 0.42 ), DG: 28.95 (SD: 3.07). With ANOVA test, PCOS model indicated a significant difference with Estrus, Metestrus and Diestrus (p = 0.000).The average of Collagen – 4 expression P: 41.60 (SD: 3.36 ), PG: 22.40 (SD: 3.51), EG: 21.40 (SD: 1.52), MG: 23.80 (SD: 1.92), DG: 23.80 (SD: 1.79) with ANOVA test indicated a significant difference (p = 0.00). The decrease of MMP-9 activity and the increase of TIMP-1 activity occuring in PCOS model have resulted in the increase of Collagen 4 expression and finally caused the thickening fibrous follicle wall consequently, the failure of follicle enlargement may mechanically occur.

Keyword :

PCOS, , PCOS model, , MMP-9 activity, , TIMP-1 activity, , Collagen-4,

References :

Beloosesky R, Gold R and Almog B,(2004) . Induction of polycystic ovary by testoterone in immature female rats: Modulation of apoptosis and attenuation of glucose/ insulin ratio, Inter. J. mol. med : Inter. J. mol. med

Cardenas H, Pope WF,,(1997) Administration of testosterone from day 13 of the oestrous cycle to oestrus increased the number of corpora lutea and conceptus survival in gilts. J Amin Sci : J Amin Sci

Henmi H, Endo T, and Nagsawa K,,(2001) Lysyl Oxidase and MMP-2 Expression in Dehydroepiandrosterone-Induced Polycystic Ovary in Rats, Biol. Reprod : Biol. Reprod

S lowey MJ,,(2001) Polycystic Ovary Syndrome: New Perspective on an Old Problem. Southern Medical Journal : Southern Medical Journal

Archive Article

Cover Media Content

Volume : 13 / No. : 1 / Pub. : 2011-01
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  2. Bioactivity Human Menopause Gonadotrophin (hmg) And Hmg Deglycosylated (hmgdg) Of Urine Of Postmenopause Woman Toward Cleavage Embryo Of Cattle In Vitro
  3. Extracellular Signal Regulated Kinase (erk1/2), Reseptor Estrogen (er) A, And Insuline Like Growth Factor I (igf-ir) Expressions On Osteoblast In Influencing Bone Density In The Female Young Rat (rattus Norvegicus) After Exercise Training
  4. The Role Of Endonuclease-g For Amniotic Cell Apoptosis Biomarker Determination In Pregnancies With Premature Rupture Of The Membrane
  5. The Microdeletion Of Rbm And Daz Genes In Male Partner Of Infertile Couple In Population With Inbreeding
  6. Compartion Study Between Animal Model Sopk And Normally Clycle Is Mmp-9, Timp-1 Enzym Activities And Collagen Expression (an Experiment Study In Rat)
  7. Characterization And Isolation Of Specific Protein From Excretory-secretory (es) Material Of L2 Dormant Of Toxocara Cati For The Diagnostic Development Of Toxocariasis By Elisa Tehnique