Detail Article


ISSN 1412-1433

Vol. 13 / No. 1 / Published : 2011-01

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Original Article :

The role of endonuclease-g for amniotic cell apoptosis biomarker determination in pregnancies with premature rupture of the membrane

Author :

  1. Benedictus Triagung Ruddy Prabantoro*1
  2. Prajitno Prabowo*2
  3. Ni Made Mertaniasih*3
  4. Fedik Abdul Rantam*4
  1. RSK St. Vincentius a Paulo (RKZ Surabaya)
  2. Fakultas Universitas Airlangga
  3. Fakultas Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

The aim of this study was to identify whether there is an association between endonuclease-G and the apoptosis of amniotic cells in pregnancies with premature rupture of the membranes. Through research study on the amnion cell apoptosis mechanism in pregnancies with premature rupture of the membranes, it is possible to know whether endonuclease-G is the biomarker of amnion cells apoptosis involved. Methods: Analytic observational methods with cross-sectional study have been used in this study design. Sample tissues from the ruptured amniotic membrane of 41 pregnant women with premature rupture of the membranes were taken and processed to be analyzed further; TUNEL methods were used for obtaining the apoptosis index analysis of amnion tissue; Western-Blotting methods were used in both the analysis of endonuclease-G (30 kDa/full length) expression and also the analysis of caspase-3 (35 kDa/full length) expression. Data, consisting age of pregnancies, endonuclease-G, caspase-3 and apoptosis index were analyzed using the chi- square test. The results of the study showed that apoptosis was significantly associated with the high level detection of endonuclease-G(p = 0.000) but was not associated with caspase-3 significantly (p = 0.316). It was also found that the apoptosis in premature rupture of the membranes in pregnancy is not dependent to the age of pregnancies. Therefore, there is an indication that the occurrence of ptosis is dominant through the caspase-independent pathway (endonuclease-G).Hence it can be concluded from the results of this study that high level detection of endonuclease-G is a biomarker of apoptosis of amnion cells in pregnancies with premature rupture of the membranes.

Keyword :

Endonuclease-G, Caspase-independent, PROM/PPROM,

References :

A badi A,(1999) Radang selaput ketuban dan plasenta serta interleukin-6 dalam air ketuban sebagai faktor penentu terjadinya persalinan pada persalinan kurang bulan membakat surabaya : Disertasi. Program Pascasarjana Ilmu Kedokteran-Universitas Airlangga

F ortunato SJ, Menon R, Bryant C, Lombardi SJ,(2000) Programmed cell death (apoptosis) as a possible pathway to metalloproteinase activation and fetal membrane degradation in premature rupture of membranes american : J Obstetrics & Gynecology

Archive Article

Cover Media Content

Volume : 13 / No. : 1 / Pub. : 2011-01
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  4. The Role Of Endonuclease-g For Amniotic Cell Apoptosis Biomarker Determination In Pregnancies With Premature Rupture Of The Membrane
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