Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 2 / Published : 2011-04

Order : 5, and page :94 - 101

Related with : Scholar   Yahoo!   Bing

Original Article :

Detection of cholera toxin-producing vibrio cholerae aquatic isolates in kalimas river-surabaya

Author :

  1. Narwati *1
  1. Health Polytechnic Indonesian Ministry of Health Surabaya

Abstract :

The purpose of this observational study is to detect cholera toxin producing Vibrio cholerae in aquatic environment of Kalimas River, Surabaya. Besides for calculate the bacterial density, abiotic factor, as temperature, power of Hydrogen, and salinity in the six points of collection were also examined.  The water samples were collected from six points along Kalimas River during rainy season (February to March 2009), and then put into an enrichment media (alkaline peptone water), filtered by 0,45 µm pore size filter, and then streaked on to TCBS agar. Drop plate method was used to calculate the bacterial density in the water samples. As many as 1.5 ml of water samples were used for preparing template DNA for PCR method. DNA extraction was done to confirmed ctxA gene by PCR method. Other parameters namely temperature, pH, and salinity were measured accordingly. This study showed that Vibrio cholerae and Vibrio alginolyticus occur in six-point of water sample collection, point 4 of collection, contains Vibrio alginolyticus only. These study, none of the V. cholerae isolates harbours ctxA gene. The temperature ranged from 27.92 degree C to 28.17 degree C, power of Hydrogen from 6.83-6.95 and salinity from 0.00395%-0.0047% and there are no statistically different (p-value > 0,05) in the abiotic parameters tested. It is concluded that Vibrio cholerae isolated in this study are not belong to cholera toxin producing Vibrio cholerae. However, it is considered necessary to characterize determinant factor(s) in expression of virulence gene and to study dissemination of the CT gene-negative non-01 strains but harbour toxR gene regulating the enterotoxic factor(s) to assess the public health significance of the CT gene-negative non-01 strains distributed in the environment.

Keyword :

toxigenic Vibrio cholerae, temperature, pH, salinity, river water,

References :

Brooks GF., Butel JS and Morse SA,(2001) Jawetz, Melnick, & Adelberg's Medical Microbiology USA : McGraw-Hill Companies

Mahalanabis D., Molla A and Sack D,(1992) Clinical Management of Cholera New York : Plenum Medical Book Company

Miller C., Feachem R., Drasar BS., et al. ,(1986) The impact of physico-chemical stress on the toxigenicity of Vibrio cholerae London : J Hyg

Archive Article

Cover Media Content

Volume : 47 / No. : 2 / Pub. : 2011-04
  1. Immunogenity Of 32.2 Kda Hemagglutinin Bm Protein Of Escherichia Coli Pili Isolated From Infertile Males' Semen
  2. Role Of Ehsp 72 On The Increase Of Il-6 And Nk Cells In Participants Of Majelis Dzikir
  3. Effect Of 2-methoxyethanol On Sperm Count, Leucocyte And Epididymal Epithelial Cell Structure In Rats (rattus Norvegicus)
  4. Reversin Increase The Plasticity Of Bone Marrow-derived Mesenchymal Stem Cell For Generation Of Cardiomyocyte In Vitro
  5. Detection Of Cholera Toxin-producing Vibrio Cholerae Aquatic Isolates In Kalimas River-surabaya
  6. Enzymatic Antioxidant Protection In Physical Exercise With Active And Passive Interval
  7. Review Article: Improving The Quality Of Western Blot Result
  8. Green Clover Potentiates Delaying The Increment Of Imbalance Bone Remodeling Process In Postmenopausal Women
  9. Knee Pain Relieved. The Use Of Extra Anatomy Pathway
  10. Nutritional Condition In Hemodialysis Patients. Differences Between Groups In Surabaya, Indonesia And Juntendo University, Japan
  11. Etiology And Risk Factors For Community Acquired Pneumonia In Dr. Zainoel Abidin Hospital, Banda Aceh
  12. Serological Survey On Avian Influenza Subtype H5n1 Viral Antibody In Healthy Blood Donor In Surabaya
  13. Effect Of Topical Hyaluronate And Freeze-dried Amnion Membrane Administration On Ck 16 Protein Expression And The Number Of Epithelial Layers In Superficial Wound Of Male Wistar Strain Rats