UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 2 / Published : 2011-04

Order : 3, and page :81 - 87

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Original Article :

Effect of 2-methoxyethanol on sperm count, leucocyte and epididymal epithelial cell structure in rats (rattus norvegicus)

Author :

  1. Alfiah Hayati*1
  2. Mustaejah*2
  3. Jean*3
  4. Siti Nuzulul Maskurotin*4
  5. Ratri Anjasari*5
  1. Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya
  2. Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya
  3. Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya
  4. Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya
  5. Department of Biology, Faculty of Science and Technology, Airlangga University, Surabaya

Abstract :

2-Methoxyethanol (2-ME) is one of the toxic agent from phatalate ester groups that potentially affect the structure of spermatozoa. This poison is a liquid, volatile, almost odorless, and enters the body through the skin, inhaled or food. In mammals, 2-ME can affect the structure and function of the testes, and is involved in the regulation of apoptosis of primary spermatocytes and testicular lesions. This experiment using 30 mice was to analyze the quality of sperm and epididymis epithelial cell apoptosis test. 2-ME dose 200 mg/kg, frequency and exposure duration of 2-ME was varied (1, 3 and 6 times/week). The animals in treated group (n = 30) were divided into two groups, consisting of treatment and control groups. Each group was divided into three sub-groups of treatment (n = 5). Subgroup treated rats were given subcutaneous injections of 0.2 ml 200 mg/kg/2-ME with exposure frequency varies, while control subgroups were injected with physiological saline solution in the same frequency. Results showed the number of spermatozoa in control and treatment groups were not significantly different (p > 0.05). The number of leukocytes increased in all treatment groups (p <0.05). Some of the epididymis epithelial cells of the treated group stained with HE showed purple (dark) color and had a nucleus smaller than normal. Apoptosis was detected in all treatment groups with the DAB staining of the epididymis epithelial cells in the treatment group, which appeared brown (dark) and showed shrinking core green cytoplasm. Green cytoplasm was seen in normal epithelial cells. In conclusion, exposure to 2-ME as much as 200 mg/kg/day increases the number of leukocytes and apoptotic cells in the epididymis.

Keyword :

2-methoxyethanol, spermatozoa, leukocyte, epididymal epithel, apoptosis,


References :

Barone F., S. Aguanno and A. D`Agostino,(2005) Modulation of MAA-induced Apoptosis in Male Germ Cells : Role of Sertoli Cells P/Q-type Calcium Channels - : Reproductive Biology and Endocrinology

Chandrasekaran Y. and JH. Richburg,(2005) The p53 Protein Influences the Sensitivity of Testicular Germ Cells to Mono-(2-Etylhexyl) Phtalate-Induced Apoptosis by Increasing the Membrane Levels of Fas and DR5 and Decreasing the Intracellular Amount of c-FLIP - : Biology of reproduction

Hayati A., Yunaida B., I.B. Rai Pidada, Darmanto W., dan Winarni D.,(2004) Efek 2-methoxyethanol terhadap struktur histologi testis mencit - : Penelitian Hayati

Hayati A., Any Devi, and I.B. Rai Pidada,(2005) Spermatozoa Motility and Morphological Recovery Process in Mice after The Induction of 2- Methoxyethanol - : Folia Medica Indonesiana

Hess RA,(2000) Effects of Environmental Toxicants on the Efferent duct, epididymis and Fertility - : Journal of Reproduction an Fertility Supplement<



Archive Article

Cover Media Content

Volume : 47 / No. : 2 / Pub. : 2011-04
  1. Immunogenity Of 32.2 Kda Hemagglutinin Bm Protein Of Escherichia Coli Pili Isolated From Infertile Males' Semen
  2. Role Of Ehsp 72 On The Increase Of Il-6 And Nk Cells In Participants Of Majelis Dzikir
  3. Effect Of 2-methoxyethanol On Sperm Count, Leucocyte And Epididymal Epithelial Cell Structure In Rats (rattus Norvegicus)
  4. Reversin Increase The Plasticity Of Bone Marrow-derived Mesenchymal Stem Cell For Generation Of Cardiomyocyte In Vitro
  5. Detection Of Cholera Toxin-producing Vibrio Cholerae Aquatic Isolates In Kalimas River-surabaya
  6. Enzymatic Antioxidant Protection In Physical Exercise With Active And Passive Interval
  7. Review Article: Improving The Quality Of Western Blot Result
  8. Green Clover Potentiates Delaying The Increment Of Imbalance Bone Remodeling Process In Postmenopausal Women
  9. Knee Pain Relieved. The Use Of Extra Anatomy Pathway
  10. Nutritional Condition In Hemodialysis Patients. Differences Between Groups In Surabaya, Indonesia And Juntendo University, Japan
  11. Etiology And Risk Factors For Community Acquired Pneumonia In Dr. Zainoel Abidin Hospital, Banda Aceh
  12. Serological Survey On Avian Influenza Subtype H5n1 Viral Antibody In Healthy Blood Donor In Surabaya
  13. Effect Of Topical Hyaluronate And Freeze-dried Amnion Membrane Administration On Ck 16 Protein Expression And The Number Of Epithelial Layers In Superficial Wound Of Male Wistar Strain Rats