Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 2 / Published : 2011-04

Order : 4, and page :88 - 93

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Original Article :

Reversin increase the plasticity of bone marrow-derived mesenchymal stem cell for generation of cardiomyocyte in vitro

Author :

  1. Budi Susetyo Pikir*1
  2. Helen Susilowati*2
  3. Eryk Hendrianto*3
  4. Fedik Abdulrantam*4
  1. Department of Cardiology & Vascular Medicine - Faculty of Medicine Airlangga University, Surabaya
  2. Stem Cell Group – Institute of Tropical Disease Airlangga University, Surabaya
  3. Stem Cell Group – Institute of Tropical Disease Airlangga University, Surabaya
  4. Departement of Virology - Faculty of Veterinary Medicine Airlangga University, Surabaya

Abstract :

Low efficiency of stem cell treatment is a big problem in the treatment of stem cell in Acute Myocardial Infarction. Transdifferentiation of Bone marrow-derived mesenchymal stem cells into cardiomyocyte took long time in experimental tissue culture. The purpose of the study is to speed up transdifferentiation of bone marrow-derived stem cells into cardiomyocyte in vitro by inducing dedifferentiation of bone marrow-derived mesenchymal stem cell, before induction by 5-aza-2’-deoxycytidine into cardiomyocyte. In this study two-three months old 2.5 kg weight adult male New Zealand Rabbits were anethezied with ether, thigh bones were excised, and bone marrow cells were obtained by aspiration. In our experiments after 1 week of mesenchymal stem cell cultures, 20 nM reversin was given to induce dediferentiation and after 24 hours exposure with 9 mM 5-aza-2-deoxycytidine, early phase of cardiomyocyte differentiation was appeared as cultured cell were strongly positive for GATA-4 and weakly positive for MLC-2a, although beating cardiomyocyte has not yet been appeared at the end of experiment. In these experiment also showed a marked CD34+ and c-kit+ gene expression on RT-PCR examination. In conclusion, reversine increase plasticity of bone marrow-derived mesenchymal stem cell to generate cardiomyocyte after 5-aza-2’-deoxycytidine induction. CD34+ and c-kit+ may be a marker for cardiomyocyte progenitor cells

Keyword :

bone marrow-derived mesenchymal stem cells, cardiomyocyte, reversine, plasticity, 5-aza-2’-deoxycytidine,

References :

Fukuda K, and Yuasa S,(2006) Stem Cells as a source of Regenerative Cardiomyocytes - : Circ Res

Heng BC, Haider HK, Sim EK-W et al,(2004) Strategies for directing the differentiation of stem cells into the cardiomyogenic lineage in vitro - : Cardiovasc Res

Limana F, Zacheo A, Mocini D, et al,(2007) Identification of Myocardial and Vascular Precursor Cells in Human and Mouse Epicardium - : Circ Res

Makino S, Fukuda K, Miyoshoi S, et al,(1999) Cardiomyocytes can be generated from bone marrow stromal cells in vitro - : J Clin Invest

Planat-Benard V, Menard C, Andre M, et al. ,(2004) Spontaneous Cardiomyocyte differentiation from Adipose Tissue Stroma Cells - : Circ Res

Archive Article

Cover Media Content

Volume : 47 / No. : 2 / Pub. : 2011-04
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  2. Role Of Ehsp 72 On The Increase Of Il-6 And Nk Cells In Participants Of Majelis Dzikir
  3. Effect Of 2-methoxyethanol On Sperm Count, Leucocyte And Epididymal Epithelial Cell Structure In Rats (rattus Norvegicus)
  4. Reversin Increase The Plasticity Of Bone Marrow-derived Mesenchymal Stem Cell For Generation Of Cardiomyocyte In Vitro
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