Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 2 / Published : 2011-04

Order : 7, and page :108 - 111

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Original Article :

Review article: improving the quality of western blot result

Author :

  1. Viskasari Pintoko Kalanjati*1
  1. Department of Anatomy and Histology, Faculty of Medicine Airlangga University

Abstract :

The western blot is one of useful techniques in detecting specific protein in the given sample, other than the immunohistochemistry and ELISA (enzyme-linked immunosorbent assay). When probed against the recombinant target protein as the standard sample, it can be used to analyze the exact densitometry value of target protein in the sample, whilst probing against the protein-pool sample or house-keeping protein samples are relatively quantified the densitometry value of the target protein (Buckley et al. 2006). Many factors may affect the western blot results, and here we will discuss about how to trouble-shoot some of these difficulties to increase the quality the results.

Keyword :

immunoblotting, trouble-shooting,

References :

Buckley ST, Foley PF, Innes DJ, et al.,(2006) GABAA receptor b isoform protein expression in human alcoholic brain: Interaction with genotype - : Neurochem International

Harlow E, Lane D,(1988) Immunoblotting--Immunohistochemistry - : Cold Spring Harbour Laboratory

Sambrook J, Fritsch EF, Maniatis T,(1989) Molecular cloning. A laboratory manual New York : Cold Spring Harbor

Archive Article

Cover Media Content

Volume : 47 / No. : 2 / Pub. : 2011-04
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