UNIVERSITAS AIRLANGGA



Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 47 / No. 2 / Published : 2011-04

Order : 7, and page :108 - 111

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Original Article :

Review article: improving the quality of western blot result

Author :

  1. Viskasari Pintoko Kalanjati*1
  1. Department of Anatomy and Histology, Faculty of Medicine Airlangga University

Abstract :

The western blot is one of useful techniques in detecting specific protein in the given sample, other than the immunohistochemistry and ELISA (enzyme-linked immunosorbent assay). When probed against the recombinant target protein as the standard sample, it can be used to analyze the exact densitometry value of target protein in the sample, whilst probing against the protein-pool sample or house-keeping protein samples are relatively quantified the densitometry value of the target protein (Buckley et al. 2006). Many factors may affect the western blot results, and here we will discuss about how to trouble-shoot some of these difficulties to increase the quality the results.

Keyword :

immunoblotting, trouble-shooting,


References :

Buckley ST, Foley PF, Innes DJ, et al.,(2006) GABAA receptor b isoform protein expression in human alcoholic brain: Interaction with genotype - : Neurochem International

Harlow E, Lane D,(1988) Immunoblotting--Immunohistochemistry - : Cold Spring Harbour Laboratory

Sambrook J, Fritsch EF, Maniatis T,(1989) Molecular cloning. A laboratory manual New York : Cold Spring Harbor





Archive Article

Cover Media Content

Volume : 47 / No. : 2 / Pub. : 2011-04
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  4. Reversin Increase The Plasticity Of Bone Marrow-derived Mesenchymal Stem Cell For Generation Of Cardiomyocyte In Vitro
  5. Detection Of Cholera Toxin-producing Vibrio Cholerae Aquatic Isolates In Kalimas River-surabaya
  6. Enzymatic Antioxidant Protection In Physical Exercise With Active And Passive Interval
  7. Review Article: Improving The Quality Of Western Blot Result
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  10. Nutritional Condition In Hemodialysis Patients. Differences Between Groups In Surabaya, Indonesia And Juntendo University, Japan
  11. Etiology And Risk Factors For Community Acquired Pneumonia In Dr. Zainoel Abidin Hospital, Banda Aceh
  12. Serological Survey On Avian Influenza Subtype H5n1 Viral Antibody In Healthy Blood Donor In Surabaya
  13. Effect Of Topical Hyaluronate And Freeze-dried Amnion Membrane Administration On Ck 16 Protein Expression And The Number Of Epithelial Layers In Superficial Wound Of Male Wistar Strain Rats