Detail Article

Folia Medica Indonesiana

ISSN 0303-7932

Vol. 39 / No. 1 / Published : 2003-01

Order : 9, and page :40 - 44

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Original Article :

Stimulatory effect of bradykinin on the activity of cyclooxygenase and prostaglandin production in culture fibroblasts

Author :

  1. Sri Agus Sudjarwo*1
  1. Department of Medical Pharmacology Airlangga University School of Medicine, Surabaya

Abstract :

The effect of bradykinin on cyclooxygenase (COX) activity and Prostaglandin (PGE2) production were investigated in cultured fibroblasts. Bradykinin significantly induced PGE2 synthesis in mouse fibroblast in a dose-dependent manner. The bradykinin stimulated PGE2 release was apparent at concentrations of 0.5 μM, 1μM and 2 μM. The bradykinin concentration achieving a maximum effect at 1μM. We next examined which of the COX enzymes was expressed in bradykinin-stimulated fibroblasts by using western blotting analyses. As a result, western blotting manifested activation of COX-1 was more potent than COX-2 polypeptide at position of 70 kDa in response to 1μM bradykinin. The present study suggests that mechanism of bradykinin increase PGE2 production in culture fibroblast is mainly attributed to the action of COX-1 and COX-2, respectively.

Keyword :

Bradykinin, cyclooxygenase, prostaglandin, fibroblast,

References :

Burch RM and Axelrod,(1987) Dissociation of bradykinin-induced prostaglandin formation from phosphattidylinositol turnover in swiss 3T3 fibroblasts: evidence for G protein regulation of phospholipase A2 - : Proc Natl Acad Sci USA

Chomczynski P and Sacchi N,(1987) Single-step method of RNA isolation by acid guanidinium thiocyanatphenol- chloroform extraction - : Anal Biochem

Archive Article

Cover Media Content

Volume : 39 / No. : 1 / Pub. : 2003-01
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