Journal of Basic Medical Veterinary
ISSN 2302-6820
Vol. 4 / No. 1 / Published : 2015-06
Order : 2, and page :7 - 12
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Original Article :
The effect combination of guava leaf (psidium guajava l.) and sambiloto leaf (andrographis paniculata) on total and diffcount leucocyte of mice (mus musculus) exposed to heat stress
Author :
- Andi Rulita Hadi*1
- Dewa Ketut Meles*2
- Rudy Sukamto Setiabudi*3
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
This research was conducted to determine the effect of combination of guava leaf (Psidium guajava L.) and sambiloto leaf (Andrographis paniculata) extract in maintaining the body immunity against heats stress exposure. Flavonoid active compound consisted in guava leaf (Psidium guajava L.) and andrographolide in sambiloto leaf (Andrographis paniculata) are substances that serve as immunomodulator. The experimental animals used are 30 male mice with 25 gram average body weight. The treatment were divided into 5 groups and each group got 6 repetitions. (K-) as control was treated with vehivulum, P1 was treated with combination of 75% guava and 25% sambiloto extract, P2 was treated with combination of 50% guava and 50% sambiloto extract, P3 was treated with combination of 25% guava and 75% sambiloto extract. Each treatment was given perorally with the dose of 0,5 ml/mice/day. The treatment were given for 10 days and were exposed to heat stress on 8 until 10 days. The parameters used total and leucocyte count shown in each treatment. The experimental design used in this study was completely random design (CRD). The data were analized using ANOVA (Analysis of Variance) continued with honesty significant difference test with significance of 5%. The result showed that there is significant effect from the therapy on the total and leucocyte count especially to neutrofil, limfosit and monosit. The treatment from P3 group showed the best result in maintaining the total of leucocyte in mice exposed to heat stress.
Keyword :
guava, sambiloto, leucocyte, heat stress,
References :
Bouchama, A. and Knochel J.P. ,(2002) Heart Stroke. 346 (25): 1978-1998. : New England Journal Medicine.
Chao, W. dan B.F. Lin. ,(2010) Isolation and Indetification of Bioactive Compounds in Androgrphis pani¬culata (Chuanxinlian). 5: 1-15. : Chin. Med. J.
Lakshmi,(2009) Screening of Psidium guajava Leaf Extracts for Antistress Activity in Different Experimental Animal Models. 1(6): 359-366. : Pharmacognosy research,
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