Jurnal Ilmiah Biologi FST
ISSN 2303-3428
Vol. 1 / No. 1 / Published : 2013-01
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Original Article :
Motilitas spermatozoa mencit (mus musculus) setelah pemberian polisakarida krestin dari ekstrak jamur coriolus versicolor
Author :
- Sri Puji Astuti Wahyuningsih*1
- Jauharotus Shobahah*2
- Alfiah Hayati*3
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Abstract :
This study aimed to know the motility speed of sperm mice (Mus musculus) after using polysaccharide crestine of Coriolus versicolor mushroom extract. Twenty four male mice aged 8-10 week, 25-30 g weight were used as experimental animals which were divided into four groups (1 controlled group and 3 treatment groups) which consisted of 6 animals for each. The first group was the controlled group (P0) that aquades 0,1 mL was daily put by gavage for 62 days. On the other hand, the treatment groups (P1, P2, and P3) were treated by giving polysaccharide crestine derived from Coriolus versicolor mushroom extract with 3 different doses, such as: 1,5; 3,0; dan 6,0 mg/kg BB for 62 days. The sperms were collected from epididymis cauda. The parameters of a given sperm quality were motility speed. Further, the data were analyzed by employing one-way ANOVA. This study resulted that the sperm motility speed of controlled group was higher; that was, (5,487 µm/second) compared to the three treated groups; they were in order: 4,768; 4,345; and 4,197 µm/second. The conclusion could be drawn that giving polysaccharide crestine of Coriolus versicolor mushroom extract could decrease motility speed as well.
Keyword :
polysaccharide crestine, sperm motility speed, Mus musculus.,
References :
Aryosetyo, L.,(2009) Hubungan antara Jumlah Leukosit dengan Morfologi Spemratozoa pada Pasien Infertilitas - : di rumah sakit Dokter Kriadi Fakultas Kedokteran
Cui, J. dan Chisti, Y.,(2003) Polysaccharopeptides of Coriolus versicolor: physiological activity, uses, and production - : J. biotechnology advances, New Zaeland: Science Direct
Fisher, M., and Yang, L.X.,(2002) Anticancer Effects and Mechanism of Polysaccharide-K (PSK): Implication of Cancer Imunotherapy - : J. Anticancer Research, 22 (3): 1737-54
Hayati, A.,(2007) Kajian Kualitas dan Protein Membran Spermatozoa Tikus (Rattus norvegicus) Akibat Pemaparan 2-Methoxyethanol Yogyakarta : Disertasi, Universitas Gadjah Mada,
Hayati, A,(2011) Spermatologi Surabaya : pusat penerbitan dan percetakan Unair
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