Jurnal Ilmiah Biologi FST
ISSN 2303-3428
Vol. 1 / No. 1 / Published : 2013-01
Related with : Scholar Yahoo! Bing
Original Article :
Pengaruh ekstrak etanolik kecambah kacang hijau (phaseolus radiatus) terhadap jumlah dan morfologi spermatozoa mencit yang terpapar 2-methoxyethanol
Author :
- Citra Maruliyananda*1
- Alfiah Hayati*2
- I.B. Rai Pidada*3
- -
- -
- -
Abstract :
Male infertility causes by many factor, one of them is toxic chemical 2-methoxyethanol (2-ME). The Phaseolus radiatus containt the antioxidant vitamin E. Vitamin E as antioxidant can avoid free radicals and break the chain of lipid peroxidation process in cell membrane. The ethanol extract of Phaseolus radiatus used to overcome the effect of 2-ME. This main aim of this study to know the influence of this extracts to sperm count and sperm morphology of mice after exposure of 2-ME. Thirty BALB/C male mice (Mus musculus) 8-9 week old, weighed 25-28 grams, was divided into 5 groups, 2 control groups and 3 treatment groups. Negative control group (Kn) were given aquades 0,1 ml by gavage within 40 days, positive control group (Kp) were given 2-ME 200 mg/kg body weight daily by intraperitoneal injection within 5 days, and continued by giving aquades 0,1 ml within 35 days, treatment groups (P1, P2, dan P3) were given 2-ME 200 mg/kg body weight daily within 5 days, continued by giving extract in three doses 0,5 ; 1 ;and 2 g/kg BB within 35 days. Mice were sacrified, epididimis were collected and examined. Count and normal morphology of spermatozoa were observed. The data analyzed by One-way ANOVA to investigate the influenced of treatment groups. Then LSD test to show the differences mean between treatment groups. The result showed that 2-ME can decrease sperm count and sperm normal morphology. The extract can increase sperm count and normal morphology after exposure of 2-ME. The conclution of this study, the extract at dose of 1 g/kg can increased sperm sperm count, whereas the dose of 2 g/kg can increase normal morphology of sperm. The green bean sprout ethanol extract at dose of 2 g/kg can recover sperm count and normal morphology of sperm.
Keyword :
2-ME, Phaseolus radiatus, spermatozoa,
References :
Hayati, A., D.R. Any, dan I.B. Rai Pidada,(2005) Spermatozoa Motility and Recovery Process in Mice after The Induction of 2-Methoxyethanol Surabaya : Folia Medica Indonesiana, 41 (2): 90-95.
Hayati A., Yunaida B., I.B. Rai Pidada, Darmanto W., dan Winarni D,(2004) Efek 2-methoxyethanol terhadap Struktur Histologi Testis Mencit (Mus musculus) Surabaya : Penelitian Berkala Hayati, 10 (1):7-12.
Astawan M,(2005) Kacang Hijau: Antioksidan yang Membantu Kesuburan Pria, - : http://web.ipb.ac.id/~tpg/de/pubde_ntrtnhlth_kacanghijau.php.
Anggrahini, S.,(2009) Pengaruh Lama Pengecambahan terhadap Kandungan α-Tokoferol dan Senyawa Proksimat Kecambah Kacang Hijau (Phaseolus radiatus L.). - : http://patpijogja.wordpress.com/2009/08/27/pengaruh-lama pengecambahan-terhadap-kandungan-a-tokoferol-dan-senyawaproksimat-kecambah-kacang-hijau-phaseolus-radiatus-l-oleh-srianggrahini-staf-pengajar-fakultas-teknologi-pertanian-ugm/.
Hariyatmi.,(2004) Kemampuan vitamin E seb
Archive Article
Cover Media | Content |
---|---|
![]() Volume : 1 / No. : 1 / Pub. : 2013-01 |
|