UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Biologi FST

ISSN 2303-3428

Vol. 1 / No. 1 / Published : 2013-01

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Original Article :

Pengaruh ekstrak etanolik kecambah kacang hijau (phaseolus radiatus) terhadap jumlah dan morfologi spermatozoa mencit yang terpapar 2-methoxyethanol

Author :

  1. Citra Maruliyananda*1
  2. Alfiah Hayati*2
  3. I.B. Rai Pidada*3
  1. -
  2. -
  3. -

Abstract :

Male infertility causes by  many factor,  one  of them is toxic chemical  2-methoxyethanol  (2-ME). The Phaseolus radiatus  containt the antioxidant vitamin E. Vitamin E as antioxidant can avoid free radicals  and break the chain of lipid peroxidation process in cell membrane. The ethanol extract of  Phaseolus radiatus used to overcome the effect of 2-ME. This main aim of this  study to know the influence of  this  extracts to sperm  count and sperm morphology of mice  after exposure of 2-ME.  Thirty  BALB/C male mice (Mus musculus) 8-9 week old, weighed 25-28 grams, was divided into 5 groups, 2 control groups and 3 treatment groups.  Negative control group (Kn) were given aquades 0,1 ml by gavage within 40  days, positive control group (Kp)  were given  2-ME 200 mg/kg  body weight daily by intraperitoneal  injection within 5 days,  and continued by giving  aquades 0,1 ml  within  35  days, treatment groups (P1, P2, dan P3) were given  2-ME  200 mg/kg body weight daily within 5 days, continued by giving extract in three doses 0,5 ; 1 ;and 2 g/kg BB  within  35 days. Mice were sacrified, epididimis were collected and examined. Count  and normal morphology  of spermatozoa were observed. The data analyzed by One-way ANOVA to investigate the influenced of treatment groups.  Then LSD test  to show  the differences  mean  between treatment groups. The result showed that 2-ME can decrease sperm count and sperm normal morphology. The extract can increase sperm count and  normal morphology  after exposure of 2-ME. The conclution of this study,  the  extract  at  dose of  1  g/kg  can increased sperm  sperm count, whereas the dose of  2  g/kg  can  increase  normal morphology of sperm. The green bean sprout ethanol extract at dose of 2 g/kg can recover sperm count and normal morphology of sperm.

Keyword :

2-ME, Phaseolus radiatus, spermatozoa,


References :

Hayati, A., D.R. Any, dan I.B. Rai Pidada,(2005) Spermatozoa Motility and Recovery Process in Mice after The Induction of 2-Methoxyethanol Surabaya : Folia Medica Indonesiana, 41 (2): 90-95.

Hayati A., Yunaida B., I.B. Rai Pidada, Darmanto W., dan Winarni D,(2004) Efek 2-methoxyethanol terhadap Struktur Histologi Testis Mencit (Mus musculus) Surabaya : Penelitian Berkala Hayati, 10 (1):7-12.

Astawan M,(2005) Kacang Hijau: Antioksidan yang Membantu Kesuburan Pria, - : http://web.ipb.ac.id/~tpg/de/pubde_ntrtnhlth_kacanghijau.php.

Anggrahini, S.,(2009) Pengaruh Lama Pengecambahan terhadap Kandungan α-Tokoferol dan Senyawa Proksimat Kecambah Kacang Hijau (Phaseolus radiatus L.). - : http://patpijogja.wordpress.com/2009/08/27/pengaruh-lama pengecambahan-terhadap-kandungan-a-tokoferol-dan-senyawaproksimat-kecambah-kacang-hijau-phaseolus-radiatus-l-oleh-srianggrahini-staf-pengajar-fakultas-teknologi-pertanian-ugm/.

Hariyatmi.,(2004) Kemampuan vitamin E seb



Archive Article

Cover Media Content

Volume : 1 / No. : 1 / Pub. : 2013-01
  1. Pengaruh Penggunaan Matras Elektromagnetik Terhadap Toleransi Glukosa Pada Tikus Putih (rattus Novergicus) Diabetik Tipe Ii
  2. Pengaruh Pemberian Polisakarida Krestin Dari Ekstrak Coriolus Versicolor Terhadap Kadar Hemoglobin Pada Mencit Yang Terpapar Timbal
  3. Uji Toksisitas Subkronik Polisakarida Krestin Dari Ekstrak Coriolus Versicolor Terhadap Kadar Kreatinin Mus Musculus
  4. Motilitas Spermatozoa Mencit (mus Musculus) Setelah Pemberian Polisakarida Krestin Dari Ekstrak Jamur Coriolus Versicolor
  5. Analisis Keanekaragaman Tanaman Kana (canna Sp.) Berdasarkan Karakter Morfologi
  6. Efek Imunostimulatori Beberapa Fraksi Teripang Lokal Phyllophorus Sp Terhadap Histologi Limpa Mencit (mus Musculus) Yang Diinfeksi Mycobacterium Tuberculosis
  7. Uji Efektivitas Ekstrak Kulit Buah Jeruk Purut (citrus Hystrix D.c.) Dan Jeruk Kalamondin (citrus Mitis Blanco) Sebagai Biolarvasida Nyamuk Aedes Aegypti L.
  8. Uji Efektivitas Ekstrak Daun Jeruk Purut (citrus Hystrix D.c) Dan Daun Jeruk Kalamondin (citrus Mitis Blanco) Sebagai Biolarvasida Terhadap Kematian Larva Instar Iii Nyamuk Aedes Aegypti L
  9. Keanekaragaman Morfologi Bunga Pada Chrysanthemum Morifolium Ramat Dan Varietasnya
  10. Pengaruh Ekstrak Etanolik Kecambah Kacang Hijau (phaseolus Radiatus) Terhadap Jumlah Dan Morfologi Spermatozoa Mencit Yang Terpapar 2-methoxyethanol
  11. Pengaruh Ekstrak Teripang Lokal Phyllophorus Sp. Terhadap Diameter Germinal Center Limpa Mencit (mus Musculus) Yang Diinfeksi Mycobacterium Tuberculosis
  12. Pengaruh Periode Subkultur Terhadap Kadar Saponin Akar Rambut Tanaman Ginseng Jawa (talinum Paniculatum Gaertn.)
  13. Induksi Akar Dari Eksplan Daun Ginseng Jawa (talinum Paniculatum Gaertn.) Dengan Zat Pengatur Tumbuh Auksin Secara In Vitro
  14. Uji Toksisitas Subkronik Polisakarida Krestin Dari Ekstrak Coriolus Versicolor Terhadap Kadar Sgpt Mus Musculus L.