UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Biologi FST

ISSN 2303-3428

Vol. 1 / No. 1 / Published : 2013-01

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Original Article :

Pengaruh ekstrak teripang lokal phyllophorus sp. terhadap diameter germinal center limpa mencit (mus musculus) yang diinfeksi mycobacterium tuberculosis

Author :

  1. I.B. Rai Pidada*1
  2. Dimas Putra Leksana*2
  3. Dwi Winarni*3
  1. -
  2. -
  3. -

Abstract :

This research aimed  to find out the potential sea cucumber  Phyllophorus sp.extract as an immunostimulant to spesific  immune response against M.  tuberculosis infected.  Therefore  this research used 36 male mice, BALB-C strain,  aged  3–4  month and weighted  30-40 g divide into 6 groups. Negative control group K(-) was given with solvent, without  M.  tuberculosis infected, positive control group K(+)  was given with solvent,  M.  tuberculosis  infected,treatment group (P) was given with Phyllophorus sp.  extract,  M.  tuberculosis infected.  Sea cucumber  Phyllophorus sp.  extract was given continously for 14 days. Dose given equal to 0,0462 g dry/ kg BW/day. Each treatment group divided into 2 subgroup : Sub group A,  infected  with  M.    tuberculosis    on day 15 after treatment and sacrificed on day 18, while sub group B: there is group which is infected by M.  tuberculosis 2 times, on day 15 and also on day 18, they  sacrificed on day 28.  The solvent used was  CMC 0,5%. The amount of  M.  tuberculosis suspension which is infected through  intraperitoneal was 106 CFU.  The spleen was taken at the end of treatment and histological preparations made.  Diameter of splenic germinal center are used as an indicator  of least potentially local sea cucumber Phyllophorus sp. as an immunostimulant  to specific immune response. Data were analyzed using ANOVA followed Duncan test at α = 0,05. The results showed that based on changes in the  spleen  germinal center  diameter  of  M.  tuberculosis  infected mice,  sea cucumbers Phyllophorus sp.  extract was    not potential as an immunostimulant to specific immune response.

Keyword :

Phyllophorus sp., Mycobacterium tuberculosis, germinal center, immunostimulant,


References :

Aprelina, D.L.,(2011) Pemanfaatan Teripang Lokal Phyllophorus sp. untuk Meningkatkan Imunitas Tubuh Terhadap Infeksi Mycobacterium tuberculosis Berdasarkan Indikator Kadar Interleukin-12. Surabaya : Skripsi. Universitas Airlangga.

Aminin, Agafonova, I., Kalnim, V., Silchenko, A., Avilov, S., Stonile, V, Collin, P., and Woodward,,(2004) Immonomodulatory Property of Frondoside A a Major Triterpene Glycoside from Sea Cucumber - : Journal of Medicinal Food, 1(3): 443-445

Abbas, A. K. dan A. H. Lichtmann.,(2005) Cellular and Molecular Immunology 5 th edition. - : Elvesier

Baratawidjaja, K.G.,(2006) Imunologi Dasar. Jakarta : Edisi ke-7 Fakultas Kedokteran Universitas Indonesia.

Bellanti, A.J.,(1993) Imunologi Umum. Edisi ke-3 Yogyakarta. : Gadjah Mada University Press. Yogyakarta. Hal 39-41, 647





Archive Article

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Volume : 1 / No. : 1 / Pub. : 2013-01
  1. Pengaruh Penggunaan Matras Elektromagnetik Terhadap Toleransi Glukosa Pada Tikus Putih (rattus Novergicus) Diabetik Tipe Ii
  2. Pengaruh Pemberian Polisakarida Krestin Dari Ekstrak Coriolus Versicolor Terhadap Kadar Hemoglobin Pada Mencit Yang Terpapar Timbal
  3. Uji Toksisitas Subkronik Polisakarida Krestin Dari Ekstrak Coriolus Versicolor Terhadap Kadar Kreatinin Mus Musculus
  4. Motilitas Spermatozoa Mencit (mus Musculus) Setelah Pemberian Polisakarida Krestin Dari Ekstrak Jamur Coriolus Versicolor
  5. Analisis Keanekaragaman Tanaman Kana (canna Sp.) Berdasarkan Karakter Morfologi
  6. Efek Imunostimulatori Beberapa Fraksi Teripang Lokal Phyllophorus Sp Terhadap Histologi Limpa Mencit (mus Musculus) Yang Diinfeksi Mycobacterium Tuberculosis
  7. Uji Efektivitas Ekstrak Kulit Buah Jeruk Purut (citrus Hystrix D.c.) Dan Jeruk Kalamondin (citrus Mitis Blanco) Sebagai Biolarvasida Nyamuk Aedes Aegypti L.
  8. Uji Efektivitas Ekstrak Daun Jeruk Purut (citrus Hystrix D.c) Dan Daun Jeruk Kalamondin (citrus Mitis Blanco) Sebagai Biolarvasida Terhadap Kematian Larva Instar Iii Nyamuk Aedes Aegypti L
  9. Keanekaragaman Morfologi Bunga Pada Chrysanthemum Morifolium Ramat Dan Varietasnya
  10. Pengaruh Ekstrak Etanolik Kecambah Kacang Hijau (phaseolus Radiatus) Terhadap Jumlah Dan Morfologi Spermatozoa Mencit Yang Terpapar 2-methoxyethanol
  11. Pengaruh Ekstrak Teripang Lokal Phyllophorus Sp. Terhadap Diameter Germinal Center Limpa Mencit (mus Musculus) Yang Diinfeksi Mycobacterium Tuberculosis
  12. Pengaruh Periode Subkultur Terhadap Kadar Saponin Akar Rambut Tanaman Ginseng Jawa (talinum Paniculatum Gaertn.)
  13. Induksi Akar Dari Eksplan Daun Ginseng Jawa (talinum Paniculatum Gaertn.) Dengan Zat Pengatur Tumbuh Auksin Secara In Vitro
  14. Uji Toksisitas Subkronik Polisakarida Krestin Dari Ekstrak Coriolus Versicolor Terhadap Kadar Sgpt Mus Musculus L.