Jurnal Ilmiah Biologi FST
ISSN 2303-3428
Vol. 1 / No. 1 / Published : 2013-01
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Original Article :
Pengaruh periode subkultur terhadap kadar saponin akar rambut tanaman ginseng jawa (talinum paniculatum gaertn.)
Author :
- Aila Ikhtimami*1
- Hery Purnobasuki*2
- Y. Sri Wulan Manuhara*3
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Abstract :
The aim of this study was to determine the effect of subculture period on dry weight and saponin content of ginseng jawa (Talinum paniculatim Gaertn.) hairy root. This study used transformated hairy root of T.paniculatum leaf explants which has been inserted by T-DNA plasmid of Agrobacterium rhizogenes and the media were semisolid MS medium without plant growth regulators as subculture medium. The roots were treated by subculture periods (0, 2, 3, 4 weeks) and were cultured for 10 weeks. Datas were obtained as fresh weight root, dry weight root, and saponin content at the end of subculture period. Dry weight data analysis used one-way ANOVA followed up with LSD test (5% significance level). Saponin content were analyzed by semi-quantitative descriptive with Thin Layer Chromatography (silica gel GF254 ). The results showed that the treatment of various subculture periods had significant influence in increasing dry weight of hairy root and 2-weeks subculture period treatment produced the highest dry weight of hairy root with 0,0229±0,0031 g dry weight. The result of saponin content on Thin Layer Chromatography test showed that the treatment of various subculture periods had effect on saponin content of hairy root and the highest saponin content obtained in without subculture (0 week) treatment with spot width 34±2,83 mm 2 /0,04 dry weight. The conclusion of this study were the treatment of various periods of subculture had significant effect on the T. paniculatum hairy root dry weight with the highest dry weight obtained at 2-weeks subculture period treatment with 0,0229±0,0031 g dry weight and various of subculture periods had effect on saponin content of T. paniculatum hairy root, the highest saponin content obtained in without subculture treatment with spot width 34±2,83 mm 2/0,04 dry weight.
Keyword :
Hairy root, ginseng jawa (Talinum paniculatum Gaertn.), subculture, saponin,
References :
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Volume : 1 / No. : 1 / Pub. : 2013-01 |
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