UNIVERSITAS AIRLANGGA



Detail Article

Majalah Obstetri & Ginekologi

ISSN 0854-0381

Vol. 21 / No. 2 / Published : 2013-05

Order : 5, and page :71 - 76

Related with : Scholar   Yahoo!   Bing

Original Article :

Expressions of growth differentiation factor-9 (gdf-9) of bovine cumulus-oocyte complex in peritoneal fluid culture of infertile patients with endometriosis

Author :

  1. Lila Dewata*1
  2. Hendy Hendarto*2
  3. Mira Myrnawati*3
  4. Widjiati*4
  1. Dosen Fakultas Kedokteran
  2. Dosen Fakultas Kedokteran
  3. Mahasiswa Fakultas Kedokteran
  4. Dosen Fakultas Kedokteran Hewan

Abstract :

The decrease of GDF-9 production may impair oocyte-granulosa cell communication and lead to failure of fertilization and embryonic development. The objective of this study was to study expression of growth differentiation factor-9 (GDF-9) cumulus-oocyte complex in peritoneal culture of infertile patients with endometriosis. Peritoneal fluid were collected from infertile patients undergoing diagnostic laparoscopy and classified into non-endometriosis, mild endometriosis, and severe endometriosis according to laparoscopic finding. Cumulus-oocyte complex (COC) were aspirated from antral follicle derived from bovine ovary from local abattoirs. COC were then placed in tissue culture medium-199 (TCM-199) and divided into four group of cultere. First group contain TCM-199 as a medium served as control group, while second, third, and four groups medium were added accordingly with 3% of peritoneal fluid of non endometriosis, mild endometriosis, and severe endometriosis. These four groups were incubated in 5% CO2 at 38oC for 20 hours and COC were eventually fixated in object glasses, stained by immunocytochemistry, and followed by measurement of GDF-9 expression. Statistic analysis using Kruskal Wallis test showed differences of GDF-9 expression in four culture groups. Mann-Whitney test revealed that GDF-9 expression in endometriosis group (mild and severe) were significantly lower compared with control and non-endometriosis group and expression in severe endometriosis group were significantly lower than in mild endometriosis one (p < 0.05). In conclusion,GDF-9 expressions of bovine COC cultured in peritoneal fluid of infertile patients with endometriosis were lowered compared with that of control and non-endometriosis group and this decrease was in proportion with severity of endometriosis.(MOG 2013;21:71-76)

Keyword :

GDF-9 expression, cumulus-oocyte complex, infertility, endometriosis, peritoneal fluid,


References :

Arici A, Mulayim N,(1999) The relevance of the peritoneal fluid in endometriosis-associated infertility - : Hum Reprod

D’hooghe TM, Vodolazkala A, Kyama C, Mwenda JM and Simoens S,(2008) Health economics of endometriosis in Endometriosis Victoria : Blackwell publishing





Archive Article

Cover Media Content

Volume : 21 / No. : 2 / Pub. : 2013-05
  1. Radiotherapy Response Of Advanced Squamous And Adenocarcinoma Types Of Cervical Cancer At Dr Soetomo Hospital Surabaya
  2. Radiotherapy Outcome In Stage Iib Cervical Cancer Patients Resistant To Neoadjuvant Chemotherapy At Dr. Soetomo Hospital 2006 – 2010
  3. Correlation Between Risk Factors And Pelvic Organ Prolapse In Gynecology Outpatient Clinic, Dr. Soetomo Hospital Surabaya, 2007 – 2011
  4. Visualization Of Endometrial And Uterine Cavity Structure Abnormality With Transvaginal Sonography, Color Doppler Transvaginal Sonography And Saline Infusion Sonography In Abnormal Uterine Bleeding
  5. Expressions Of Growth Differentiation Factor-9 (gdf-9) Of Bovine Cumulus-oocyte Complex In Peritoneal Fluid Culture Of Infertile Patients With Endometriosis
  6. Viral Load Pada Kanker Serviks Terinfeksi Human Papilloma Virus Tipe 16
  7. Comparison Of Anti-mullerian Hormone (amh), Basal Follicle Stimulating Hormone (fsh), Estradiol And Antral Follicles Count As Predictors Of Ovarian Response In In Vitro Fertilization Program

Information MOG