OVOZOA
ISSN 2302-6464
Vol. 4 / No. 2 / Published : 2015-10
Order : 10, and page :126 - 131
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Original Article :
Identifikasi kualitas semen dan morfometri spermatozoa kambing marica sebagai dasar pembuatan semen beku
Author :
- U’un Pramesthi*1
- Sri Mulyati*2
- M. Gandul A.Y.*3
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
The aim of this research was to identificate the quality of fresh semen and sperm morphometry of Marica goat. Semen was collected twice a week using artificial vagina from a mature Marica goat. The research was descriptive and presented in the form of pictures and tables. Parameter observed were macroscopic test (volume, smell, colour, consistency, and pH) and microscopic test (motility, concentration, life percentage spermatozoa and percentage abnormality spermatozoa). Sperm morphometry were measured by Nikon motorized microscope with Nis software after applying Eosin-Negrosin staining technique. Each sperm was measured for four primary spermatozoa head dimensional parameters length (L), width (W), Area (A), perimeter (P), two flagell parameters middle piece (MP), End Piece (EP), and total sperm length. The result showed that sperm morphometry of Marica goat were identified L= 8.27±0.20 µm, W= 4.06±0.13 µm, A= 29.96±0.52 µm2, P= 21.31±0.34 µm, MP= 12.27±0.29 µm, EP= 39.28±0.33 µm, total sperm length= 59.84±0.59 µm. In conclusion, quality of fresh semen and sperm morphometry of Marica goat were good and deserve to be frozen semen for insemination.
Keyword :
Frozen semen, quality of semen, sperm morphometry, marica goat.,
References :
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Husin, N., T. Suteky, dan Kususiyah.,(2007) Uji Kualitas Semen Kambing Nubian dan Peranakannya Serta Kambing Boer Berdasarkan Lama Penyimpanan. 2(2): 57-66. : Jurnal Sain Peternakan Indonesia
Marti, J.I., I.M. Aparicio, C.L. Leal and M.G. Herreros.,(2012) Seasonal Dynamics of Sperm Morphometric Subpopulation and Its Association With Sperm Quality Parameters in Ram Ejakulates. 78: 528-541. : Theriogenology
Purwasih, R., Y.S. Ondho dan Sutopo.,(2013) Efektifitas Prefreezing Semen Sapi Jawa Sebagai Parameter Processing Semen Beku. 2(1): 44 – 50. : Animal Agriculture Journal
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