UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 5 / No. 2 / Published : 2016-10

Order : 2, and page :77 - 81

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Original Article :

Pengaruh penambahan perasan buah labu kuning (cucurbita moschata) dalam diluter terhadap integritas membran plasma spermatozoa sapi simmental post thawing

Author :

  1. Nur Hidayatin Ni’mah*1
  2. Dr. Sri Mulyati, drh., M.Kes.*2
  3. Nanik Sianita Widjaja, drh., S.U.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

       The aim of this study was to determine the effect of pumpkin juice (Cucurbita moschata) addition in diluter on plasma membrane integrity of simmental spermatozoa post-thawing. This study used semen from healthy bull with good libido. Each sample was divided into five group of treatments; control (-) simply diluter without pumpkin juice (Cucurbita moschata) addition, control (+) diluter mixed with 0,005 ml β-carotene 0.0002 %, (P1) diluter mixed with 1 ml of pumpkin juice 10%, (P2) diluter mixed with 1 ml of pumpkin juice 20%, and (P3) diluter mixed with 1 ml of pumpkin juice 30%. All of treatments were processed into frozen semen then followed by evaluation of plasma membrane integrity post-thawing. Obtained data were analyzed using ANOVA and continued by Duncan test. Result showed that addition of pumpkin juice in diluter can improve plasma membrane integrity of bull sperm, there were significant differences (p<0.05). Mean and deviation result showed that P3 (pumpkin juice 30%) as the highest value in maintaining plasma membrane integrity among treatments, which was 29.8 ± 4.2. It can be concluded that addition of pumpkin juice in concentration 20% is effective in maintaining spermatozoa plasma membrane integrity of simmental bull post-thawing.

Keyword :

pumpkin, plasma membrane integrity, spermatozoa,


References :

Aires V.A., K.D. Hinsch., F.M. Scholoesser., K. Bogner., S.M. Schloesser and E. Hinsch. ,(2003) In vitro and In Vivo Comparison of Egg Yolk-based and Soybean Lecithin Based Extenders For Cryopreservation of Bovine semen. 60(2) : 269-279. : Therigenology.

Arifiantini, I., T. L. Yusuf dan N. Graha.,(2005) Longivitas Dan Recoveryrate Pasca Thawing Semen Beku Sapi Fresian Holstein Menggunakan Bahan Pengencer Yang Berbeda. 29 (2) 234-241. : Buletin Peternakan.

Chatterjee, I., A.Richmond., E.Putiri., D.C.Shakes and A.Singson.,(2001) The Caenorhabditis Elegans Spe-38 Gene Encodes A Novel Four-Pass Integral Membrane Protein Required For Sperm Function At Fertilization. 132: 2795–27113. : Development.

Feradis. ,(2009) Peranan Antioksidan Dalam Pembekuan Semen. 6(2): 63 -70. : Jurnal Peternakan.

Pommer, A.C., J.Rutlant and S.A Mayes.,(2003) Phosphorylation of Protein Tyrosine Residues in Fresh and Cryopservaed stallion Spermatozoa Under Capacitating Condition. 68:12



Archive Article

Cover Media Content

Volume : 5 / No. : 2 / Pub. : 2016-10
  1. Efektifitas Hormon Prostaglandin F2α (pgf2α) Dan Hormon Gonadotropin Terhadap Gambaran Ovarium Dankadar Progesteron Tikus Putih (rattus Norvegicus) Dalam Teknik Sinkronisasi Birahi Dan Superovulasi
  2. Pengaruh Penambahan Perasan Buah Labu Kuning (cucurbita Moschata) Dalam Diluter Terhadap Integritas Membran Plasma Spermatozoa Sapi Simmental Post Thawing
  3. Penambahan L-arginin Dalam Pengencer Susu Skim Kuning Telur Terhadap Viabilitas Dan Motilitas Spermatozoa Sapi Limousin Post Thawing Pada Semen Beku
  4. Pengaruhpemberian Pasta Tomat (solanum Lycopersicum) Terhadap Jumlah Sel Spermatogonium Pada Testis Mencit (mus Musculus) Yang Dipapar Boraks
  5. Persentase Membran Plasma Utuh Dan Nekrosis Spermatozoa Sapi Limousin Post Thawing Setelah Penambahan L-arginin Berbagai Konsentrasi Dalam Pengencer Skim Kuning Telur
  6. Pengaruh Pemberian Ekstrak Daging Buah Mahkota Dewa (phaleria Macrocarpa) Terhadap Kualitas Spermatozoa Tikus Putih (rattus Norvegicus)
  7. Hubungan Paritas Dan Umur Terhadap Indeks Fertilitas Sapi Perah Peranakan Frisian Holstein (pfh) Di Koperasi Unit Desa (kud) Sri Wigati Pagerwojo Tulungagung
  8. Pengaruh Penembakan Laserpunktur Pada Titik Reproduksi Itik Mojosari Jantan Terhadap Berat Dan Volume Testis
  9. Pengaruh Ph Lendir Serviks Saat Inseminasi Buatan Terhadap Pesentase Kebuntingan (conception Rate) Pada Sapi Perah Di Dataran Tinggi Dan Dataran Rendah
  10. Pengaruh Temperatur Rektum Dan Temperatur Vagina Saat Inseminasi Buatan Terhadap Angka Kebuntingan Pada Sapi Perah Di Dataran Tinggi Dan Dataran Rendah
  11. Pengaruh Temperatur Tubuh Saat Inseminasi Buatan Tehadap Angka Kebuntingan Sapi Perah Di Dataran Tinggi Dan Dataran Rendah Di Kabupaten Pasuruan Jawa Timur
  12. Kemampuan Peternak Mendeteksi Birahi Sapi Potong Di Kecamatan Wringin Kabupaten Bondowoso
  13. Gambaran Histopatologi Tubulus Seminiferus Mencit (mus Musculus) Yang Terpapar Brucella Suis
  14. Pengaruh Imunisasi Dengan Whole Bakteri Brucella Abortus S19 Terhadap Jumlah Sel Spermatogenik Pada Testis Kelinci (orytolagus Cuniculus)

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