UNIVERSITAS AIRLANGGA



Detail Article

OVOZOA

ISSN 2302-6464

Vol. 5 / No. 2 / Published : 2016-10

Order : 5, and page :93 - 98

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Original Article :

Persentase membran plasma utuh dan nekrosis spermatozoa sapi limousin post thawing setelah penambahan l-arginin berbagai konsentrasi dalam pengencer skim kuning telur

Author :

  1. Mitha Ardila Rahmawati*1
  2. Dr. Suherni Susilowati, drh., M.Kes.*2
  3. Prof. Dr. Koesnoto Supranianondo, drh., M.S.*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This research was determined percentage membrane integrity and necrosis spermatozoa Limousine bull post thawing after adding L-Arginine with different concentrations in extender skim milk and egg yolk to increased frozen semen quality. This research was a laboratory experimental with Complete Random Design, consisted of four treatments and six replications. The first treatment P0 no L-Arginine added as control. P1 was treated with 0,004 M L-Arginine. P2 was treated with 0,005 M L-Arginine. P3 was treated with 0,006 M L-Arginine. The data was analysed by Analysis of Variant (ANOVA) and continued with Duncan method. The result showed that determine significant different between treatments. The post thawing membrane integrity’s result was (P0) 34,37a ± 2,56, (P1) 35,12a ± 2,59, (P2) 38,10ab ± 4,77, and (P3) 40,85b ± 3,82. The post thawing necrosis’s result was (P0) 55,56b ± 6,53, (P1) 52,90b ± 8,04, (P2) 45,21a ± 4,97, and (P3) 41,10a ± 3,94. The optimum concentration added L-Arginine to increased membrane integrity and decreased necrosis spermatozoa in this research was 0,006 M.

Keyword :

Limousine bull, spermatozoa, L-Arginine, membrane integrity, necrosis,


References :

AL-Ebady, A.S., S.O. Hussain, K.I. AL-Badry, and F.F. Ibrahim.,(2012) Effect of Adding L.arginine on Some Parameters of Bull Sperms After Freezing in Liquid Nitrogen (-196oC). 11(2): 15-161. : AL-Qadisiya Journal of Vet.Med.Sci.

AL-Ebady, A.S., S.O. Hussain, K.I. AL-Badry, and F.F. Ibrahim.,(2012) Effect of Adding Arginine in Different Concentrations on Some Physical Properties of Poor Motile Bull Sperms During Different Months. Jurnal of Veterinary Medicine and Animal Health. 4(9): 130-135. : Jurnal of Veterinary Medicine and Animal Health.

Chatterjee, S. and Gagnon C.,(2001) Production of Reactive Oxygen Spesies by Spermatozoa Undergoing Cooling, Freezing, and Thawing. 59: 451-458. : Mol Reprod Dev.

Revell, S.G., and Mrode R.A.,(1994) An Osmotic Resistance Test for Bovine Semen. 36: 77-86 : Anim Reprod Sci

Rizal, M., dan Herdis,(2010) Peranan Antioksidan Dalam Meningkatkan Kualitas Semen Beku. 20(3): 139-145. : Wartazoa

Srivastava, S., Prashant D.,



Archive Article

Cover Media Content

Volume : 5 / No. : 2 / Pub. : 2016-10
  1. Efektifitas Hormon Prostaglandin F2α (pgf2α) Dan Hormon Gonadotropin Terhadap Gambaran Ovarium Dankadar Progesteron Tikus Putih (rattus Norvegicus) Dalam Teknik Sinkronisasi Birahi Dan Superovulasi
  2. Pengaruh Penambahan Perasan Buah Labu Kuning (cucurbita Moschata) Dalam Diluter Terhadap Integritas Membran Plasma Spermatozoa Sapi Simmental Post Thawing
  3. Penambahan L-arginin Dalam Pengencer Susu Skim Kuning Telur Terhadap Viabilitas Dan Motilitas Spermatozoa Sapi Limousin Post Thawing Pada Semen Beku
  4. Pengaruhpemberian Pasta Tomat (solanum Lycopersicum) Terhadap Jumlah Sel Spermatogonium Pada Testis Mencit (mus Musculus) Yang Dipapar Boraks
  5. Persentase Membran Plasma Utuh Dan Nekrosis Spermatozoa Sapi Limousin Post Thawing Setelah Penambahan L-arginin Berbagai Konsentrasi Dalam Pengencer Skim Kuning Telur
  6. Pengaruh Pemberian Ekstrak Daging Buah Mahkota Dewa (phaleria Macrocarpa) Terhadap Kualitas Spermatozoa Tikus Putih (rattus Norvegicus)
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