UNIVERSITAS AIRLANGGA



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BIOSAINS

ISSN 1412-1433

Vol. 14 / No. 2 / Published : 2012-05

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Original Article :

Analysis the influence of time and detergen washing to dna from cement liquids spotted in fga locus, d21s11 and dys19 using str-pcr method

Author :

  1. Indah Nuraini*1
  2. Soekry Erfan Kusuma*2
  3. Agung Sosiawan*3
  1. Lembaga Penyakit Tropis Universitas Airlangga
  2. Fakultas Kedokteran Universitas Airlangga
  3. Fakultas Kedokteran Gigi Universitas Airlangga

Abstract :

Sex abuse cases, especially by murdering or robbering from year to year have an increasingly tendency. A test which’s absolutly donein some cases of sex abuse is the evidence of sperm. In some cases, cement liquids spotted were found in the condition which havebeen changed because of intentionaly washing by detergent to DNA of cement liquids spotted or even it’s not like soaked by water. Itcomplicates the identifi cation process and untill now, the research about the infl uence of washing by detergent to DNA of cement liquidsspotted is not much known yet. The objective of this study was to analysis cement spot on the effect of time duration and washing bydetergent to FGA LOCUS as a forensic molecular identifi cation material. The individual identity can be commonly decided using DNAprofi ling which is common used to identifi cate is Short Tandem Repeat (STR) by using 13–20 locuses. The focus of this research is togasp the effect of the time duration (1, 7, 30, 60, and 90 days) and washing by detergent to FGA locus, D21S11, DYS19 as a forensicmolecular identifi cation material. The result of this DNA identifi cation showed that the infl uence of the time duration and washingby detergent to cement liquids spotted, longer time of action made the decreasing of DNA rate. It’s showed from the result of KruskalWallis analysis with p = 0.000 (p < 0.05). Nevertheless, the decreasing of DNA rate in forensic sample will not much signifi cantlyinfl uent to the successful of DNA profi ling, commonly in Short Tandem Repeat (STR) test. It’s happened because the DNA rate whichis needed in STR and PCR tests kindly just a little (1–25 ng/ul), so that the risk of failure in amplifi cation process is very small, withthe DNA’s not totally degraded as an assential . The visualization results were analyzed descriptively, by seeing the presence of theribbons or bands based on the size of product (base pair) in each spotted of cement liquids. In this research found that even thoughthere’s DNA rate of cements spotted reduction but the result of STR-PCR of FGA Locus, D21S11 and Y chromosom in DYS19 locusstill can be all positively detected.

Keyword :

Cement liquids spotted, , the influence of detergen washing to DNA, , STR-PCR,


References :

Chadha PD,(1995) Ilmu forensik dan toksikologi jakarta : Ilmu forensik dan toksikologi

Kusuma SE,(2007) Forensik Molekuler. Buku Ajar Ilmu Kedokteran Forensik dan Medikolegal, edisi ketiga, Ed. Hariadi A, Hoediyanto. Surabaya : Departemen Ilmu Kedokteran Forensik dan Medikolegal Fakultas Kedokteran Universitas Airlangga,

Notosoehardjo,(1993) Pengaruh Rendaman Bercak Semen manusia dalam Bermacam-macam Deterjen Pada Test Zink dan Tes Fosfatase asam. Surabaya : Majalah Ilmu Kedokteran Forensik

Sosiawan A,(2007) Analisis Efek Paparan Panas Suhu Ekstrim Tinggi Terhadap DNA Yang Berasal Dari Tulang dan Gigi. Surabaya : Program Pascasarjana Universitas Airlangga

Yudianto A, ,(2007) Efektivitas tes asam fosfatase dan tes zink pada pemeriksaan bercak semen manusia sebagai identitas primer. Surabaya : Departemen Ilmu Kedokteran Forensik dan Medikolegal Fakultas Kedoteran Universitas Airlangga,





Archive Article

Cover Media Content

Volume : 14 / No. : 2 / Pub. : 2012-05
  1. Analysis Of Myoc Gene Polymorphism Among Malay Race Patients With Primary Open Angle Glaucoma In Palembang
  2. Polymorphism Analysis Of Alpha–helix Coiled Coil Rod Homologue Gene Exon 2 To Patients With Psoriasis In Palembang Malay Race
  3. Isolation And Identification Of High Cellulolytic Activity Of Bacteria From Golden Snail’s (pomacea Canaliculata) Gut
  4. Biocompability Of Low Dose Of Human Menopause Gonadotropin(hmg) And Deglycosilation Of Human Menopause Gonadotropine (dghmg) Against Bovine Eggs
  5. Profile Of Narcotic Evidence From Black Circulation Market In Law Police Area East Java Using Gc-ms Method
  6. Analysis The Influence Of Time And Detergen Washing To Dna From Cement Liquids Spotted In Fga Locus, D21s11 And Dys19 Using Str-pcr Method
  7. The Analyze Of Human Dna Soft Tissue That Contaminated Formalin During 1 Month And 6 Month At Locus D13s317 Using Str-pcr

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