UNIVERSITAS AIRLANGGA



Detail Article

BIOSAINS

ISSN 1412-1433

Vol. 14 / No. 3 / Published : 2012-09

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Original Article :

Identification of fertilization receptor protein (zp3) on goat zona pellucida as candidate of immunocontraceptive substance using in vitro fertilization technique

Author :

  1. Imam Mustofa*1
  2. L Mahaputra*2
  3. Yoes Prijatna Dachlan*3
  1. Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Airlangga
  2. Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Airlangga
  3. Tropical Disease Center Airlangga University.

Abstract :

he researches of immunocontraception have been done in several species, but haven’t yet been done in goat. As well as known thathormonal contraception up to now is still having side effects. In preliminary study, a crude of goat zona pellucida protein was effectiveprohibitted graviditation of mice. The aim of this study was to prove that obtained gZP3 protein isolate was representing receptor ofgoat sperm. Isolate of gZP3 was incubated on goat sperm, while antibody of gZP3 was incubated on goat oocyte, then each of them wasin-vitro fertilized separately. Antibody of gZP3 produce on rabbit (Oryctolagus cuniculus). The first immunization, gZP3 suspensionwas mixed with Complete Freund’s Adjuvant (CFA) 1:1 (v/v) followed by boosters twice with the interval of 11 days, the suspension wasmixed with Incomplete Freund’s Adjuvant (IFA) 1:1 (v/v). In in-vitro fertilization technique, antibody of gZP3 which was supplementedmedia during maturation process of goat oocytes in vitro had decreased (p < 0.05) embryos cleavage rate. Protein of gZP3 which wassupplemented in the capacitation media of goat sperms decreased (p < 0.05) of cleavage rate. Based on these results, it was concludedthat antibody gZP3 having a fertilization receptor protein at the goat zona pellucida.

Keyword :

goat zona pellucida-3, in vitro , fertilization,


References :

Abbas AK, AH. Lichtman, and JS Pober,(2003) Cellular and Molecular Immunology 5th Ed. - : WB Saunders

Alberts B, Johnson A, Lewis J, Martin R, Roberts K, and Walter P,(2002) Molecular Biology of The Cell 4th Ed. New York : Garland Science,

Aulani’am, SB Sumitro, S Hardjopranjoto, Sutiyoso dan T Soendoro,(2003) Bovine Zona Pellucida Deglycosylated (bZP3dG) and The Prospect for Imunocontraceptive Vaccine. Surabaya : Media Kedokteran Hewan





Archive Article

Cover Media Content

Volume : 14 / No. : 3 / Pub. : 2012-09
  1. The Effects Of Moderate Intensity Walking Exercise On Stem Cell Mobilization, Diferentiation And Maturation In Pbmc And Bone Remodeling Of Postmenopausal Women
  2. Identification Of Fertilization Receptor Protein (zp3) On Goat Zona Pellucida As Candidate Of Immunocontraceptive Substance Using In Vitro Fertilization Technique
  3. Osteoclast Increasing Number In Periapical Inflammation Due To Lipopolisaccharide Porphyromonas Gingivalis Induction
  4. Analysis Of Blood Product Trombocyte Concentrate In Red Cross Blood Donor Unit Surabaya
  5. Biofilm Formation By Streptococcus Uberis Associated With Chronic Intramammary Infections
  6. Antioxidant Potential Of The Extract Of Green Tea (camellia Sinensis) And Red Tea (hibiscus Sabdariffa) In Atherogenesis Process In Rats With Atherogenic Diet
  7. Seperation Of Bearing X And Y Chromosome Limosin Bull Spermatozoa By Using Percoll And Chicken Eggs Albumen

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