UNIVERSITAS AIRLANGGA



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BIOSAINS

ISSN 1412-1433

Vol. 13 / No. 3 / Published : 2011-09

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Original Article :

Influent of e. coli and leucocytes againts human sperm quality in vitro

Author :

  1. S ukarjati*1
  2. Doddy M Soebadi*2
  3. Aucky Hinting*3
  4. Sudjarwo*4
  1. Biologi, FMIPA Universitas PGRI Adi Buana Surabaya
  2. Departemen Urologi, Fakultas Kedokteran Universitas Airlangga
  3. Departemen Biomedik, Fakultas Kedokteran Universitas Airlangga
  4. Departemen Kimia Farmasi, Fakultas Farmasi Universitas Airlangga

Abstract :

Male genital tract infection has been recognized as one of the causes of infertility. Indicators of the occurrence of genital tract infection is the presence of bacteria during semen culture (bacteriospermia) and the finding of leucocyte of more than 1 million/ml semen (leucotytospermia). E. coli is the most common cause of prostatitis and epididymitis. The objective of this study was to explain the effect of E. coli and leucocyte on spermatozoa quality in vitro. This study comprised three experiments. In experiment 1 sperm was incubated with E. coli, in experiment 2 with lymphocyte, and experiment 3 with granulocyte. In those experiments, sperm motility and vitality. Spermatozoa were obtained from donor with normal spermatozoa according to WHO (1999). E. coli was obtained by culturing the semen of infertile males. Lymphocyte and granulocyte were obtained from donors’ blood. Sperm preparation was made using Percoll gradient column method. Lymphocyte isolation was performed using Histopaque 1077, and granulocyte isolation used Histopaque 1077 and 1119. Sperm motility and vitality was observed by means of conventional method with microscope. The result of this study showed that in vitro E. coli had effect on the motility (p = 0.000) and vitality(p = 0.000) of sperm. Lymphocyte had effect on the motility (p = 0.034) and vitality (p = 0.041). Granulocyte had effect on motility (p = 0.000) and vitality (p = 0.017). In conclusion, E. coli has effect on the reduction of sperm quality through E. coli adhesion to the sperm. In leukocyte, granulocyte has higher effect in the reduction of sperm quality. Sperm quality reduction by granulocyte undergoes through the mechanism of ROS production.

Keyword :

sperm quality, , E. coli, , lymphocyte, , granulocyte,


References :

A garwal A, Saleh RARA, Bedaiwy MA,,(2002) Role of Reactive Oxygen Species in The pathophysiology of Human Reproduction, - : Fertil Steril

A itken RJ,,(1993) Analysis of Lipid Peroxidation Mechanism in Human Spermatozoa, - : Molecular Reproduction and Development

A uroux MR, Jacquest L, Mathieu D, Aurer J,(1991) . Is The Sperm Bacterial ratio a Determining Factor in Impairment of Sperm Motility; An In Vitro Study in Man With E. coli - : Int. J of Andrology

Comhaire F, Verschraegen G, and Vermeulen L, ,(1980) Diagnosis of Accessory Gland infection its possible role in male infertility - : Int. J of Andrology

de Lamirande E, and Gagnon C,(1992) b, Reactive oxygen species (ROS) and human spermatozoa, II: Depletion of adenosine triphosphate - : J Androl





Archive Article

Cover Media Content

Volume : 13 / No. : 3 / Pub. : 2011-09
  1. The Role Of Tgf-b1 As Switching Isotype Regulator On Siga Secretion In Saliva
  2. Influent Of E. Coli And Leucocytes Againts Human Sperm Quality In Vitro
  3. Recombinant Gen Of Encoding B-xylosidase From Geobacillus Thermoleovorans It-08 In Phis1525 Plasmid
  4. The Potency Of Blue Light Emitting Diode (led) For Photoinactivation Of Staphylococcus Aureus Bacteria With Endogeneous Porphyrin
  5. The Effect Of Tomato (lycopersicum Pyriforme) Juice On The Prevention Of Photoaging Of The Skin As A Result From Ultraviolet-b Irradiation
  6. Regeneration Of Massive Bone Defect With Bovine Hydroxyapatite As Scaffold Of Mesenchymal Stem Cells

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