UNIVERSITAS AIRLANGGA



Detail Article

BIOSAINS

ISSN 1412-1433

Vol. 14 / No. 2 / Published : 2012-05

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Original Article :

Isolation and identification of high cellulolytic activity of bacteria from golden snail’s (pomacea canaliculata) gut

Author :

  1. M. Anam Al-Arif*1
  2. Win Darmanto*2
  3. Ni Nyoman Tri Puspaningsih*3
  4. Suwarno*4
  1. Fakultas Kedokteran Hewan Universitas Airlangga
  2. Fakultas Sain dan Teknologi Universitas Airlangga
  3. Fakultas Sain dan Teknologi Universitas Airlangga
  4. Fakultas Kedokteran Hewan Universitas Airlangga

Abstract :

Cellulase can degrade cellulose to become glucose. A large number of cellulolytic microbes are available in the animal’s gut. Theaim of this research was to isolate and identify the cellulolytic bacteria with high activity from Golden snail’s gut. Some golden snailswere surface sterilized by 70% ethanol and then washed in sterile distilled water. The entire guts were removed from the abdomenand disrupted then 1 gram of gut debris was suspended in 10 ml sterile distilled water. After serial dilution, aliquots were transferredinto CMC-solid media and incubated at 35° C for 24 hours. The growing cultures were cultivated on NA-solid media. Bacteria fromsingle colonies were repeatedly grown on CMC-solid medium and covering the Petri dishes with Congo red dye. The colonies that hadhigh degradation ability then tested to endoglucanase activity and identifi ed with biochemical and 16S-rRNA tested. The result of thisresearch is there were two high cellulolytic bacteria isolates: Burkholderia pseudomallei and Klebsiella sp.

Keyword :

Golden snail, identifi cation, cellulolytic bacteria, cellulase,


References :

Al-Arif MA, H. Setyono dan Tri-Nurhajati,(2004) Isolasi dan Karakterisasi Ensim Selulase dari Keong Emas dan Rayap sebagai Bahan Pendegradasi Selulosa. Surabaya : FKH Unair

Anand AAP, Vennison SJ, Sankar SG, Prabhu DIG, Vasan PT, Raghuraman T, Geoffrey CJ and Vendan SE,,(2010) Isolation and characterization of bacteria from the gut of Bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion. - : J. Insect Sci

Bakalidou A, P. Kampfer, M. Berchtold, T. Kuhnigk, M. Wenzel and H. Konig,(2002) Cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite Mastotermes darwiniensis. - : International Journal of Systematic and Evolutionary Microbiology

Baldani VLD and Doebereiner J,,(1980) Host-plant specifi city in the infection of cereals with Azospirillum spp. - : Soil Biol Biochem

Barraquio WL, Revilla L and Ladha JK,(1997) Isolation of endophytic diazotrophic bacteria from wetland rice.



Archive Article

Cover Media Content

Volume : 14 / No. : 2 / Pub. : 2012-05
  1. Analysis Of Myoc Gene Polymorphism Among Malay Race Patients With Primary Open Angle Glaucoma In Palembang
  2. Polymorphism Analysis Of Alpha–helix Coiled Coil Rod Homologue Gene Exon 2 To Patients With Psoriasis In Palembang Malay Race
  3. Isolation And Identification Of High Cellulolytic Activity Of Bacteria From Golden Snail’s (pomacea Canaliculata) Gut
  4. Biocompability Of Low Dose Of Human Menopause Gonadotropin(hmg) And Deglycosilation Of Human Menopause Gonadotropine (dghmg) Against Bovine Eggs
  5. Profile Of Narcotic Evidence From Black Circulation Market In Law Police Area East Java Using Gc-ms Method
  6. Analysis The Influence Of Time And Detergen Washing To Dna From Cement Liquids Spotted In Fga Locus, D21s11 And Dys19 Using Str-pcr Method
  7. The Analyze Of Human Dna Soft Tissue That Contaminated Formalin During 1 Month And 6 Month At Locus D13s317 Using Str-pcr

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