UNIVERSITAS AIRLANGGA



Detail Article

BIOSAINS

ISSN 1412-1433

Vol. 14 / No. 1 / Published : 2012-01

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Original Article :

The increased expression of l-type calcium channel, protease activating receptor-1 and heat shock protein-27 and density of intracellular calcium on animal model of new zealand rabbit myometrium that using conservative surgical procedure of surabaya suture compression modification (biomolecular study in conservative surgical management of pph due to uterine atony)

Author :

  1. Agus Sulistyono*1
  2. Agus Abadi*2
  3. Endang Joewarini*3
  4. F.M. Judajana*4
  1. Fakultas Kedokteran Universitas Airlangga
  2. Fakultas Kedokteran Universitas Airlangga
  3. Fakultas Kedokteran Universitas Airlangga
  4. Fakultas Kedokteran Universitas Airlangga

Abstract :

Uterine atony as the common cause of PPH, which causing high maternal mortality especially in under developed and developingcountries, can indeed be largely rescued by compression with conservative surgical procedure by performing Surabaya suture compressionmodifi cation (SSCM). The purpose of this study was to analyze the increasing expression of L-type calcium channel, PAR-1 and HSP-27and density of intracellular calcium on animal model of aterm pregnant New Zealand rabbit uterus. True experimental single blindedstudy was done in Airlangga University Veterinary Faculty and Brawijaya University Physiology Department from March–August2011. Twenty two samples were obtained over this study which applied on aterm pregnant rabbit uterus as animal model. Animal modelconsisted of rabbit uterus in which practiced on by SSCM in 11 samples after terminating pregnancy and removing pup and placentaas well, and the remainder of another 11 samples without SSCM as the control. The assessment of L-type calcium channel, PAR-1 andHSP-27 expression and density of intracellular calcium on myometrium sample was done respectively by immunohistochemistryand fl uoro-2 double staining method at ½, 1, 2, and 24 hours intervals. For all times intervals the increasing expression of L-typecalcium channel was observed signifi cantly in SSCM compared to control (p = 0.001; p < 0.0001; p = 0.011 and p < 0.0001), alsofor intracellular calcium density (p = 0.022; p = 0.001; p = 0.005 and p = 0.002). The study showed PAR-1 and HSP-27 expressionincreasing sharply that statistically analysis indicated both all of p similarly < 0.0001 against control. SSCM suggested stronglyincreasing some component (all variable) that absolutely required to myometrial contraction.

Keyword :

PPH, Surabaya suture compression modifi cation, L-type calcium channel, PAR-1, HSP-27 and intracellular calcium,


References :

B-Lynch C, Coker A, Lawal AH, Abu J, Cowen MJ,(1997) The B-Lynch surgical technique for the control of massive postpartum haemorrhage: an alternative to hysterectomy? Five cases reported. - : Br J Obstet Gynaecol

Bolton TB, Prestwich SA, Zholos AV, Gordienko DV,(1999) Excitation-contraction coupling in gastrointestinal and other smooth muscles - : Ann Rev Physiol

Collier ML, Thomas AP, Berlin JR,(1999) Relationship between L-type Ca2+ current and unitary sarcoplasmic reticulum Ca2+ release events in rat ventricular nyocytes. - : J Physiol

Coughlin SR,(1999) How the protease thrombin talks to cells USA : Proc Natl Acad Sci

Cunningham FG, Leveno KJ, Bloom SL, Hauth JC, Rouse DJ, Spong CY,(2010) Obstetrical Hemorrhage. In Williams Obstetrics, 23rd Ed New York-Toronto : McGraw-Hill Medical Publishing Division





Archive Article

Cover Media Content

Volume : 14 / No. : 1 / Pub. : 2012-01
  1. The Expression Of β Catenin And β4 Integrin In Aggressive And Non-aggressive Basal Cell Carcinoma
  2. Mechanism Of Hypersensitivity Pneumonitis (hp) As A Result From The Exposure Of Dust From Paddy Milling On Mice (mus Musculus) Balb/c
  3. Under Fives Nutritional Rearing Model Based On Risk Factors Approach
  4. Apoptosis Pneumocyte Type I & Ii On Lsbt Resuscitated Haemorrhagic Hypovolemic Shock Experimental Study On Rat Sprague Dawley
  5. The Effects Of Glutamine On Sucrase, Maltase, Lactase Activity And Spectrin, Clathrin Expression In Repairing Microvilli Ileum In Rats Malnutrition
  6. The Increased Expression Of L-type Calcium Channel, Protease Activating Receptor-1 And Heat Shock Protein-27 And Density Of Intracellular Calcium On Animal Model Of New Zealand Rabbit Myometrium That Using Conservative Surgical Procedure Of Surabaya Suture Compression Modification (biomolecular Study In Conservative Surgical Management Of Pph Due To Uterine Atony)

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