Journal of Basic Medical Veterinary
ISSN 2302-6820
Vol. 3 / No. 2 / Published : 2014-12
Order : 5, and page :88 - 93
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Original Article :
Antiviral effectivity extract of pomegranate rind (punica granatum l. ) against newcastle disease virus in embryonated chicken egg
Author :
- Yunita Fransiska Ola *1
- Dewa Ketut Meles *2
- Suwarno*3
- Mahasiswa Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
- Dosen Fakultas Kedokteran Hewan
Abstract :
This study aims to know the antiviral properties of the rind extract of pomegranate against ND virus in embryonated chicken egg. This research used pomegranate rind extract with each dose of 10 mg/ml, 16 mg/ml, 25 mg/ml 40 mg/ml injected alantois each treatment. Each test awaited for 5 minutes before inoculated Velogenik ND virus was inoculated 0.1 ml (107EID50) on the 11 embryonated chicken egg. After that the embryonated chicken egg were incubated for 7 days in incubator at 37°C. HA test then performed to determine the presence or absence of ND virus growth in embryonated chicken egg. Data obtained by conducting tests to determine the titer HA (Hemaglutination). The results of the test analysis Oneway Anova (Analysis of Variance) and obtain the results of p <0.05. The positive control group (P0) with P1, P2, P3, P4 showed significantly different. Ortogonal polinomial test showed there is a linear relationship between increasing doses of pomegranate rind extracts on the inhibition of virus replication in the embryonated chicken eggs. Results of the research increasing doses of rind extract of pomegranate there will be a decrease in HA titer.
Keyword :
rind extract pomegranate, Newcastle Disease, embryonated chicken egg, HAtest,
References :
Beard and Hanson. ,(1984) Disease of Poultry. Amerika : edisi 8. IoWA State University Press. Ames.USA.
Rantam, A.F.,(2005) Virologi Veteriner. Surabaya : Airlangga University Press.
Murtini, S., Murwani R., Satrija F., Malole M. . ,(2006) Penetapan Rute dan Dosis Inokulasi pada Telur Ayam Berembrio sebagai Media Uji Khasiat Ekstark Benalu Teh (Scurrula oortiana.). Bogor : Departemen Ilmu Penyakit Hewan dan Kesehatan Masyarakat Veteriner. Institut Pertanian Bogor. 137-143.
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