UNIVERSITAS AIRLANGGA



Detail Article

Journal of Basic Medical Veterinary

ISSN 2302-6820

Vol. 3 / No. 2 / Published : 2014-12

Order : 2, and page :68 - 74

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Original Article :

The inhibition effect of pluchea indica less. leaf extract to pasteurella multocida (in-vitro) by diffusion disk method

Author :

  1. Mirza Atikah Madarina Hisyam*1
  2. Didik Handijatno*2
  3. Mas’ud Hariadi*3
  1. Mahasiswa Fakultas Kedokteran Hewan
  2. Dosen Fakultas Kedokteran Hewan
  3. Dosen Fakultas Kedokteran Hewan

Abstract :

This research was to determine the effect of Pluchea indica Less. leaf extract to inhibit Pasteurella multocida growth by diffusion disk method. Eleven group of treatment were conducted (P1, P2, P3, P4, P5, P6, P7, P8, P9, P10, and P11) which were P1 as negative control using blank disk contain 0% of extract, and P2 until P11 were series of Pluchea indica leaf extract concentration from 10% concentration until 100% extract concentration .The statistical analysis were using One Way ANOVA to know the effect of treatment and continue Duncan’s Multiple Range test by Statistical Program for Social Science (SPSS) version 16.0 to know the difference among treatments. The appearance of inhibition zone diameter showed the effect of treatment (p<0.05). There was significance difference between treatment and control (0% extract), but the different among treatment it selves were not clear. Treatment P11 (100% extract concentration) showed significance different with P1 (control, 0% extract). Because of among treatment show the different but not clear the concentration used for effective concentration was 100 % extract concentration (P11).      

Keyword :

Pluchea indica, disk diffusion method, inhibition effect, Pasteurella multocida,


References :

Boyce, J.D., M. Harper, I.W. Wilkie and B. Adler. ,(2010) Pasteurella. In: Gyles, C.L., J.F. Prescott, G. Songer and C.O. Thoen. Patho-genesis of Bacterial Infection in Animals USA : 4th Edition. Wiley-Blackwell. . 325-340.

Landes, M., S. Persaud and J. Dyck. ,(2004) India’s Poultry Sector Development and prospects. Agriculture and Trade Reports. Economic Research Service. United States Washington : Department of Agriculture.

Ortez, J.H. ,(2005) Disk Diffusion Testing. In: Coyle, M.B. Manual of Antimicrobial Susceptibility Testing. Amerika : American Society for Microbiology. 5, 39 – 49.





Archive Article

Cover Media Content

Volume : 3 / No. : 2 / Pub. : 2014-12
  1. The Study Of Cross Reactivity Between H5 Clade 2.3.2 Serum And H5 Clade 2.1.3 Virus By Using Serum Neutralization Test
  2. The Inhibition Effect Of Pluchea Indica Less. Leaf Extract To Pasteurella Multocida (in-vitro) By Diffusion Disk Method
  3. Chemopreventive Effect Of Gynura Procumbens (lour) Merr, On The Histopathology Of Mammary Gland In The Rats That Was Induced 12-dimethylbenz(α)antrasen
  4. The Effect Of Meniran (phyllantus Niruri, Linn.) In Increasing The Number Of Total Leukocytes And Leukocyte Counts Of Eimeria Tenella Infected Broiler
  5. Antiviral Effectivity Extract Of Pomegranate Rind (punica Granatum L. ) Against Newcastle Disease Virus In Embryonated Chicken Egg
  6. Profile Of Bone Calcium Levels In The Rat (rattus Norvegicus) After The Repositioning Fractures Of The Femur With Tikel Balung (cissus Quadrangularis) Extract Theraphy
  7. Teat Dipping Effectiveness Using Red Betel (piper Crocatum) Leaf Boiling And Iodine On Growth Of Bacteria
  8. Influence Of Infusa Mangosteen Peel (gracinia Mangostana L) For Blood Glucose Levels Of Hyperglycemic Rats
  9. Potential Of Crude Chlorella As Antibacterial Againts Salmonella Pullorum In Vitro
  10. The Potential Of Red Roses Extract (rosa Damascena Mill) As An Antiseptic Stomatitis In Snakes (python Reticulatus) On The Total Number Leukocyte And Defferential Counting Of Leukocytes

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