UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Biologi FST

ISSN 2303-3428

Vol. 1 / No. 1 / Published : 2013-01

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Original Article :

Efek imunostimulatori beberapa fraksi teripang lokal phyllophorus sp terhadap histologi limpa mencit (mus musculus) yang diinfeksi mycobacterium tuberculosis

Author :

  1. Dwi Winarni*1
  2. Sugiharto *2
  3. Cipto Dwi Handono*3
  1. -
  2. -
  3. -

Abstract :

This research was aimed to know the immunostimulator potential of Phylloporus sp.’s extract and fraction as response of Mycobaterium tuberculosis infection and fraction has the highest potential of immunostimulator based on histological changes in germinal center’s diameter of Mus musculus. This research used 60 male mices (Mus musculus)strain BALB/C, age 3-4 months, 30-40 g, then divided into 4 groups of control and 6 groups of treatment. Negative control group (KN), positive control group (KP), polar fraction group (FP), semi-polar fraction group (FS), and non-polar fraction group (FN), then respectively divided into two group based on the day of sacrifice. KN 17, KP 17, FP 17, FS 17, and FN 17 sacrificed on day 17 with once injection of M. tuberculosis  on day  14. KN 28,KP 28, FP 28, FS 28, and FN 28 sacrificed on day 28 with the second injection on day 18. KN 17 and KN 28 was only given solvent and was not injected with M. tuberculosis. Solvents were used in the fractionation stage of Phyllophorus sp. for each group, polar group, semi-polar group, and non-polar group sequentially are n-hexane, ethyl acetate, and ethanol. The observation of germinal center’s diameter used light microscope on 400x magnification by taking 5 germinal center on one slide of spleen with 6 slices each. Germinal center’s diameter was the average of the longest and the shortest diameter from measurement. Data was then analyzed using ANOVA and Duncan test α = 0,05. The average of germinal center diameter from each group areKN17 = 58,7 µm, KP17=58,3 µm, FP17=73,1 µm, FS17=76,9 µm, FN17=103,5 µm, KN28=57,4 µm, KP28=58,6 µm, FP28=71,2µm, FS28=70,7 µm, and FN28=90,7 µm with p value  of  ANOVA=0,00.  Based on the result, the  negative control group has no difference with the positive one, but the positive control group are different if we compare it with the treatment group. This result indicated that the extract of Phyllophorus sp has the potential to be immunostimulator and the fraction which has the highest potency is non-polar fraction.

Keyword :

Phyllophorus sp. Mycobacterium tuberculosis, diameter of germinal center, polar, semi-polar and nonpolar fraction,


References :

Arifah, S. and Purwanti, O. S.,(2008) Pengaruh Pemberian Epineprin dan Hidrokortison Terhadap Jumlah dan Diameter Germinal Center Kelenjar Getah Bening Tikus Putih Jantan Wisting - : Berita Ilmu Keperawatan ISSN 1979-2697, 1(3): 67-84

Baratawidjaja, Karnen Garna,(1991) Imunologi Dasar Jakarta : Edisi ke dua Jakarta:Balai Penerbit FKUI

Baratawidjaja. ,(2006) Imunologi Dasar Jakarta : Edisi ke-7, FK Universitas Indonesia, Jakarta

Barnes P.F., and B. Wizel.,(2000) Type 1 Cytokines and Pathogenesis of Tuberculosis. - : American Journal of Respiratory and Critical Care Medicine.161: 1773-1774

Bellanti, J.,(1993) Immunologi Umum Yogyakarta : Edisi ke-3.Gadjah Mada University Press. Yogyakarta. Hal 647.





Archive Article

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Volume : 1 / No. : 1 / Pub. : 2013-01
  1. Pengaruh Penggunaan Matras Elektromagnetik Terhadap Toleransi Glukosa Pada Tikus Putih (rattus Novergicus) Diabetik Tipe Ii
  2. Pengaruh Pemberian Polisakarida Krestin Dari Ekstrak Coriolus Versicolor Terhadap Kadar Hemoglobin Pada Mencit Yang Terpapar Timbal
  3. Uji Toksisitas Subkronik Polisakarida Krestin Dari Ekstrak Coriolus Versicolor Terhadap Kadar Kreatinin Mus Musculus
  4. Motilitas Spermatozoa Mencit (mus Musculus) Setelah Pemberian Polisakarida Krestin Dari Ekstrak Jamur Coriolus Versicolor
  5. Analisis Keanekaragaman Tanaman Kana (canna Sp.) Berdasarkan Karakter Morfologi
  6. Efek Imunostimulatori Beberapa Fraksi Teripang Lokal Phyllophorus Sp Terhadap Histologi Limpa Mencit (mus Musculus) Yang Diinfeksi Mycobacterium Tuberculosis
  7. Uji Efektivitas Ekstrak Kulit Buah Jeruk Purut (citrus Hystrix D.c.) Dan Jeruk Kalamondin (citrus Mitis Blanco) Sebagai Biolarvasida Nyamuk Aedes Aegypti L.
  8. Uji Efektivitas Ekstrak Daun Jeruk Purut (citrus Hystrix D.c) Dan Daun Jeruk Kalamondin (citrus Mitis Blanco) Sebagai Biolarvasida Terhadap Kematian Larva Instar Iii Nyamuk Aedes Aegypti L
  9. Keanekaragaman Morfologi Bunga Pada Chrysanthemum Morifolium Ramat Dan Varietasnya
  10. Pengaruh Ekstrak Etanolik Kecambah Kacang Hijau (phaseolus Radiatus) Terhadap Jumlah Dan Morfologi Spermatozoa Mencit Yang Terpapar 2-methoxyethanol
  11. Pengaruh Ekstrak Teripang Lokal Phyllophorus Sp. Terhadap Diameter Germinal Center Limpa Mencit (mus Musculus) Yang Diinfeksi Mycobacterium Tuberculosis
  12. Pengaruh Periode Subkultur Terhadap Kadar Saponin Akar Rambut Tanaman Ginseng Jawa (talinum Paniculatum Gaertn.)
  13. Induksi Akar Dari Eksplan Daun Ginseng Jawa (talinum Paniculatum Gaertn.) Dengan Zat Pengatur Tumbuh Auksin Secara In Vitro
  14. Uji Toksisitas Subkronik Polisakarida Krestin Dari Ekstrak Coriolus Versicolor Terhadap Kadar Sgpt Mus Musculus L.

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