UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Biologi FST

ISSN 2303-3428

Vol. 3 / No. 1 / Published : 2015-01

Order : 56, and page :56 - 66

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Original Article :

Pengaruh intensitas cahaya terhadap biomassa dan kadar saponin kalus ginseng jawa (talinum paniculatum gaertn.) pada berbagai waktu kultur

Author :

  1. Afifatul Alwiyah,*1
  2. Y. Sri Wulan Manuhara*2
  3. Edy Setiti Wida Utami*3
  1. Mahasiswa Fakultas Sains dan Teknologi
  2. Dosen Fakultas Sains dan Teknologi
  3. Dosen Fakultas Sains dan Teknologi

Abstract :

The aims of this research was to study the effects of light intensity on callus biomass and saponin content of ginseng jawa (Talinum paniculatum Gaertn.). Callus induced from leaf explants on solid Murashige and Skoog (MS) medium supplemented by 2 mg/L of 2,4-D and 1 mg/L of kinetin. Callus induced by treatment of combination between light intensities 1000, 2000, 4000, 6000 lux, also dark condition and culture period 4, 6, and 8 weeks. Each treatment of combination was repeated 6 times. Retrieval of data were callus biomass (fresh and dry weight) and saponin content. The data of biomass were analyzed by using manova test (significance of 5%). Saponin content was analyzed semi-quantity using Thin Layer Chromatography (TLC). The result showed that the highest average of dry weight (as parameter) obtained at treatment combination 4000 lux light intensity and 8 weeks culture period was 0,0699 gram. Meanwhile the highest saponin content obtained at 4000 lux light intensity and 6 weeks culture period was 58,5 mm2/0,01 gram dry weight of callus and color intensity +4. With the results that light intensities influence on callus biomass and saponin content of ginseng jawa at different culture period.Keywords: biomass, callus, culture period, light intensity, saponin content, Talinum paniculatum Gaertn

Keyword :

biomass, , callus, , culture period, light intensity, saponin content, Talinum paniculatum Gaertn,


References :

Abe, H.,,(1980) Pharmacological actions of saikosaponins isolated from Bupleurum falcatum. Planta Medica 40: 366-372 : Effects of saikosaponin on liver function, Planta Medica 40: 366-372

Blando, F., A. P. Scardino, L. De Bellis, I. Nicoletti, and G. Giovinazzo,,(2005) Characterization of in vitro anthocyanin-producing sour cherry (Prunus cerasus L.) Food Research Internat 38: 937-942 : callus culture,

Khristiyana, L., E. Anggarwulan., dan Marsusi.,(2005) Pertumbuhan, kadar saponin, dan nitrogen jaringan tanaman daun sendok (Plantago major L.) pada pemberian asam giberelat (GA3)), Jurnal Biofarmasi 3 (1): 1693-2242 : Jurnal Biofarmasi 3 (1): 1693-2242





Archive Article

Cover Media Content

Volume : 3 / No. : 1 / Pub. : 2015-01
  1. Pengaruh Pemberian Pupuk Hayati (biofertilizer) Pada Berbagai Dosis Pupuk Dan Media Tanam Yang Berbeda Terhadap Pertumbuhan Dan Produktivitas Tanaman Tomat (lycopersicon Esculentum) Pada Polybag
  2. Studi Viabilitas Dan Pola Pertumbuhan Bacillus Megaterium Pada Konsentrasi Molase Dan Waktu Inkubasi Yang Berbeda
  3. Keanekaragaman Dan Hubungan Kekerabatan Pada Jambu Air (syzygium Aqueum Burm.f. Alston) Melalui Pendekatan Morfologi Di Perkebunan Bhakti Alam, Pasuruan
  4. Biosistematika Varietas Pada Jambu Biji (psidium Guajava L.) Melalui Pendekatan Morfologi Di Agrowisata Bhakti Alam Nongkojajar, Pasuruan
  5. Kepadatan Dan Keanekaragaman Plankton Di Perairan Mangetan Kanal Kabupaten Sidoarjo Provinsi Jawa Timur Dari Daerah Hulu, Daerah Tengah Dan Daerah Hilir Bulan Maret 2014
  6. Pengaruh Konsentrasi Sukrosa Terhadap Biomassa Dan Kadar Saponin Kalus Ginseng Jawa(talinum Paniculatum Gaertn.) Pada Berbagai Waktu Kultur
  7. Pengaruh Intensitas Cahaya Terhadap Biomassa Dan Kadar Saponin Kalus Ginseng Jawa (talinum Paniculatum Gaertn.) Pada Berbagai Waktu Kultur
  8. Studi Kelimpahan Dan Jenis Makrobenthos Di Sungai Cangar Desa Sumber Brantas Kota Batu
  9. Kajian Distribusi Dan Keberadaan Makrobenthos Dalam Hubungannya Dengan Suhu Di Aliran Sungai Air Panas Cangar Kota Batu
  10. Pengaruh Pemberian Biofertilizer Dan Jenis Media Tanam Terhadap Pertumbuhan Dan Produktivitas Tanaman Kubis (brassica Oleracea)

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