UNIVERSITAS AIRLANGGA



Detail Article

Jurnal Ilmiah Perikanan & Kelautan

ISSN 2085-5842

Vol. 2 / No. 2 / Published : 2010-11

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Original Article :

penambahan ekstender madu dalam proses penyimpanan sperma beku terhadap motilitas dan viabilitas spermatozoa ikan komet (carassius auratus auratus)   

Author :

  1. Boedi Setya Rahardja*1
  2. A. Shofy Mubarak*2
  3. Permana Sulistyo Rini *3
  1. Fakultas Perikanan dan Kelautan Universitas Airlangga-Kampus C Mulyorejo-Surabaya
  2. Fakultas Perikanan dan Kelautan Universitas Airlangga-Kampus C Mulyorejo-Surabaya
  3. Fakultas Perikanan dan Kelautan Universitas Airlangga-Kampus C Mulyorejo-Surabaya

Abstract :

The development of fish culture is strongly influenced by hatchery technology, especially in the stock of fish seed. Often, the problem arises in the stock of seeds, due to the maturation of gametes, broadstock fish sometimes do not happen simultaneously, one of alternative solution to the problem through the application of reproductive biotechnology, is preservation of sperm (Dirjennak, 2007). Preservation sperm is to optimize the male broadstock (Dirjennak, 2007). This storage process requires diluent and cryoprotectant material that could sustain sperm motility (Zaenab, 2007). Diluent material is used to reduce the activity of spermatozoa tha inhibit energy usage and prolong the life of sperm (Rustidja, 2000). The use of honey as a diluent material is expected to support the vitality of spermatozoa. Material of honey there are various combinations of materials a simple sugar and salt ions. With the combination of basic ingredients and simple sugar salt ions spermatozoa can produce an energy source so it can defend itself and can fertilize an egg cell after Cryoperservation process. The purpose of this research is to know the effect of adding extender honey in enhancing sperm motility and viability of fish comet after the freezing process and to determine the best dose of honey in the process of freezing sperm comet fish (Carassius auratus auratus). This research was conducted at the Laboratory Center for Artificial Insemination Singosari. The design use Completely Randomized Design (CRD) followed by Duncans multiple range test. Test materials in this research is sperm comet fish are packed in mini straw and stored in liquid nitrogen container with 9 treatments and 3 replications. Media diluent used was physiological NaCl is added to honey, glucose, fructose, and Tris Aminomethane. Honey dosages of the experiments were 0.3% (A), 0.4% (B), 0.5% (C), 0.6% (D) and 0.7% (E) and 0.05% glucose (KG); fructose 0.05% (KF), Tris Aminomethane (KT) and without the addition of physiological NaCl (KN). The main parameters of observed percentage of live sperm and duration of motion. The supporting parameters measured were fresh sperm concentration, percentage of live sperm fresh, long fresh sperm, pH, volume and color of sperm. The results of the research shows that the addition of honey with different dosage at  diluents material physiological NaCl gived significant effect (P <0.05) against the percentage of sperm motion and long life. Average percentage of long life and highest sperm obtained by treatment of the addition of honey 0.6% (D), ie 63.89% and 101.33 seconds. Average lowest percentage of life gained by treatment of honey 0.3% (C) that were 39.67 second while the lowest was in motion treatment control without the addition of (KN) that were 28.33 seconds.   

Keyword :

Carassius auratus auratus, sperm, honey, cryopreservation ,


References :





Archive Article

Cover Media Content

Volume : 2 / No. : 2 / Pub. : 2010-11
  1. The Use Of Sand Substrate As Media For Tiger Shrimp (penaeus Monodon) Post Larva Rearing
  2. The Production Of Superoxide From Shrimp Penaeus Monodon Hemocyte As An Stress Indicator On The Intensive Culture
  3. Isolation Of Aeromonas Hydrophila From Diseased Shrimp Penaeus Monodon Cultured In Traditional Ponds
  4. Efek Ph Pada Air Pemeliharaan Ikan Mas Cyprinus Carpio Terhadap Pertumbuhan Bakteri Aeromonas Hydrophilla
  5. Penambahan Ekstender Madu Dalam Proses Penyimpanan Sperma Beku terhadap Motilitas Dan Viabilitas Spermatozoa Ikan Komet (carassius auratus Auratus)   
  6. Penentuan Faktor Pengelolaan Tambak Yang Mempengaruhi Produktivitas Tambak Kabupaten Mamuju, Provinsi Sulawesi Barat
  7. Kultur Sel Otak Ikan Kerapu Bebek (cromileptes Altivelis) Dengan Menggunakan Media L-15, Mem Dan Tcm
  8. Efektivitas Penggunaan Whole Cell Dari Vibrio Alginolyticus Sebagai Vaksinoral Melalui Artemia Pada Benih Ikan Kerapu Tikus (chromileptes Altivelis)
  9. Isolasi Bakteri Indigen Sebagai Pendegradasi Bahan Organik Pada Media Pembenihan Ikan Lele Dumbo (clarias Sp.) Sistem Resirkulasi Tertutup
  10. Perubahan Patologi Kulit Ikan Gurami (osphronemu Gouramy) Akibat Infestasi Lernaea Cyprinacea Pada Derajat Infestasi  Yang Berbeda
  11. Pengaruh Pemberian Berbagai Jenis Pakan Terhadap Pertumbuhan Dan Tingkat Kelulushidupan Ikan Silih (macrognathus Aculeatus)
  12. Kemampuan Reproduksi Daphnia Magna Jantan Hasil Induksi Logam Berat (cd, Pb) Dan Pestisida Diazinon
  13. Penambahan Suplemen Madu Dalam Pakan Guna Meningkatkan Pertumbuhan Dan Kelulushidupan Benih Lobster Air Tawar Red Claw (cherax  Quadricarinatus)
  14. Sargassum Sp. Sebagai Biokontrol Terhadap Kandungan Logam Berat Timbal (pb) Yang Terserap Oleh Kerang Darah (anadara Granosa)
  15. Efektifitas Bakteri Pseudomonas Sebagai Pengurai Bahan Organik (protein,karbohidrat,lemak) Pada Media Air Limbah Pembenihan Ikan Lele Dumbo (clarias Sp.) Sistem Resirkulasi Tertutup
  16. Obyek Kesukaan Untuk Penempelan Telur (oviposisi)  Ektoparasit        Argulus Japonicus
  17. Kondisi Muara Porong Berdasarkan Indeks Klorofil-a  Dan Total Suspended Solid (tss)
  18. Pengaruh Pemberian Beberapa Bakteri Terhadap Kelangsungan Hidup Benih Ikan Lele Dumbo (clarias Sp)
  19. The Effect Of Using Kombucha On Crude Protein And Crude Fiber Contents In Water Hyacinth (eichornia Crassipes) Fermentation

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