UNIVERSITAS AIRLANGGA



Detail Article

Majalah Farmasi Airlangga (Airlangga Journal of Pharmacy)

ISSN 0852-1050

Vol. 6 / No. 2 / Published : 2008-10

Order : 7, and page :57 - 59

Related with : Scholar   Yahoo!   Bing

Original Article :

Determination of docosahexaenoic acid (dha) in food supplement using gas chromatography

Author :

  1. Asri Darmawati*1
  2. Mochammad Yuwono*2
  3. Ida Nursanti *3
  1. Dosen Fakultas Farmasi
  2. Dosen Fakultas Farmasi
  3. Mahasiswa Pasca Sarjana

Abstract :

Determination of Docosahexaenoic acid (DHA, C22:6 w-3) in two registered food supplement using Gas Chromatography (GC) have been performed. These food supplements were soft capsules containing natural fish oil that have been claimed ‘providing with DHA’. The aim of this study was to determine DHA content in natural fish oil soft capsules.   Sample was hydrolyzed with methanolic NaOH and converted to DHA-methyl ester using methanolic BF3 as catalyst. The obtained DHA-methyl ester was extracted with n-heptane and analyzed by GC using tricosanoic methyl ester as internal standard. The present work showed that all validation parameters obtained were fulfill to Association of Official Analytical Chemist (AOAC) criteria for methyl esters of fatty acids in oils and fats analysis, i.e. Rs>1.25, good linearity (rcalculated =0.9988, Vxo=3.83%), good precision (CV<10%) and good  accuracy (recoveries was 95,79%).  The optimum conditions of GC obtained were as follow, inlet and detector temperature was 250oC and 300oC respectively. Oven temperature was programmed at 180oC (1 minute), to be ramp at 10oC/minute to 280oC (3 minute). Helium’s flow rate was 1.1 mL/minute. The DHA contents in each capsule of sample X and sample Y were found to be=219.0mg (CV=0.88%) and 139.2mg (CV=1.91%), respectively. These DHA’s contents in each capsule were higher than that mention in their leaflet i.e. 95 mg and 120 mg, respectively.

Keyword :

Docosahexaenoic acid, DHA, food supplement, GC,


References :

Cunnif P.,(1995) Official Methods of Analysis of Association of Official Analytical Chemist (AOAC) 16th ed. Chapter 35, p 1, 10, Chapter 41, p 17-21 : AOAC International

O’Neil M.J.,(2001) The Merck Index, An Encyclopedia of Chemicals, Drugs and Biologicals 13th ed. p.3432, 3562 : Merck Research laboratories Division of Merck and Co., Inc., Whitehouse Station, NJ

The AnalystTM,(2002) Analysis Treatments Essential Fatty acids.htm oct 10,.(8000 356-1688 : http://www.restekcorp.com

Skoog, D.A.F., James H., Timothy A.N.,(1998) Principles of Instrumental Analysis 5th ed. p. 688, 701-722,A-1-A-19 : Saunders College Publihsing





Archive Article

Cover Media Content

Volume : 6 / No. : 2 / Pub. : 2008-10
  1. Influence Of Sorbitan Monostearate On Characteristics Of Salbutamol Sulphate Containing Ethylcellulose Microcapsules
  2. Profil Peresepan Obat Generik Di Beberapa Apotek Wilayah Surabaya.
  3. Profil Peresepan  Obat Di Apotek Wilayah Surabaya
  4. The Influence Of Ph On The Proteolytic Enzyme Activity Thermophiles Bacteria From Lapindo Mud*
  5. Pengaruh Asam-asam Organik Terhadap Pertumbuhan Lactobacillus Acidophilus, Lactobacillus Bulgaricus Dan Lactobacillus Casei (bakteri Asam Laktat)
  6. Determination Of Docosahexaenoic Acid (dha) In Food Supplement Using Gas Chromatography

Information detail_jurnal.php/MFA