Majalah Farmasi Airlangga (Airlangga Journal of Pharmacy)
ISSN 0852-1050
Vol. 6 / No. 2 / Published : 2008-10
Order : 6, and page :53 - 56
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Original Article :
The influence of ph on the proteolytic enzyme activity thermophiles bacteria from lapindo mud*
Author :
- Rosiana A.D*1
- Noor Erma, N.S.*2
- Isnaeni*3
- Mahasiswa Fakultas Farmasi
- Dosen Fakultas Farmasi
- Dosen Fakultas Farmasi
Abstract :
Lapindo mud sample containing thermopile bacteria that produced thermostable proteolytic enzyme has been determined. This research has also been done for determined influence of pH on proteolytic enzyme activity. The activitywas measured in unit / milliliter tyrosine. From 8 isolates, 7 isolates produced proteolysis enzyme and the chosen isolate named L.6 has been choen as the best isolate. Optimal growth and production enzyme have done to know when crude enzyme must be taken. Crude enzymes were taking at 63 hour growth. The enzyme activity test to be done used in pH 6.5 to 11.0 The result show at that optimum proteolytic enzyme activity of chosen isolate is achieved at pH range of 7.0 to 7.5 with activity the value 9.7563 x 10-3 unit / milliliter tyrosine.
Keyword :
Lapindo, thermophilic, proteolytic, pH,
References :
Jay, J.M.,(1992) Modern Food Microbiology 113-120, 179-188 : Chapman & Hall, London
Margolis, H.C., and Moreno, E.C,(1992) Composition of Pooled Plaque Fluid from Caries-free and Caries-positive Individuals Following Sucrose Exposure 71, 2-10 : Journal of Dental Research
Salminen, S., Wright, O.V., Ouwehand, A.,(2004) Lactic Acid Bacteria Microbiological and Functional Aspect 3rd ed. p. 19-43 : Marcel Dekker Inc, New York
Walsh, L.J.,(2009) Dental Plaque Fermentation and Its Role in Carries Assessment SA, 8, 1-3 : International Dentistry
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 Volume : 6 / No. : 2 / Pub. : 2008-10 |
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