UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 23 / No. 3 / Published : 2007-09

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Original Article :

The optimation of tyrosine kinase activity from sperm isolation of friesian holstein cow

Author :

  1. Sri Pantja Madyawati*1
  2. Pudji Srianto*2
  1. Bagian Reproduksi Veteriner, Fakultas Kedokteran Hewan Universitas Airlangga
    Kampus C Unair, Mulyorejo Surabaya 60115, Telp. (031) 5992785; Fax.(031)5993015,
  2. Bagian Reproduksi Veteriner, Fakultas Kedokteran Hewan Universitas Airlangga
    Kampus C Unair, Mulyorejo Surabaya 60115, Telp. (031) 5992785; Fax.(031)5993015,

Abstract :

The research purpose was to determine the optimation of tyrosine kinase activity from sperm isolation of FH cow according to pH, temperature and optimum incubation time. Fresh semen as samples were collected using artificial vagina. Tyrosin kinase was isolated by means of centrifugation and ultrasonication. Activity unit of cow sperm tyrosine kinase is the amount of fosfat µmol of transferred ATP by each mililiter tyrosine kinase enzyme per minute in optimum condition. The optimation of tyrosine kinase activity of sperm according to optimum condition included different pH (6, 6.5, 7, 7.5, 8), temperature ( 20, 25, 30, 35, 40)oC and incubation time (20, 25, 30, 35, 40) minute activity was optimum. The result of this research showed that the optimum activity tyrosine kinase of sperm at pH 7, temperature 35oC and 30 minutes incubation time with 0,0219 unit activity.

Keyword :

Tyrosine kinase, tyrosine kinase activity,


References :

Aulanni’am,(2004) Prinsip dan Teknik Analisis Biomolekul. Cetakan pertama. - : Fakultas Pertanian Universitas Brawijaya Press

ESE Hafez,(2000) Reproduction in Farm Animals. 7th Edition Philadelphia : Lea & Febiger

AL Lehninger,(1990) Dasar-dasar Biokimia Jakarta : Jilid I. Alih Bahasa: Maggy Thenawijaya. Erlangga

DW Martin,(1983) Biokimia. Review of Biochemistry. Edisi ke-19. Alih Bahasa: A. Dharma dan S.Kurniawan Jakarta : CV EGC Penerbit Buku Kedokteran

M Matsuo,(1985) Modification and identification of glutamate residues at the arginine-recognition site in the catalytic sub unit of adenosin 3’5’-cyclic monophosphate dependent protein kinase of rabbit skeletal muscle - : Biochem J. 187

M Matsuo,(1985) Modification and identification of glutamate residues at the arginine-recognition site in the catalytic sub unit of adenosin 3’5’-cyclic monophosphate dependent protein kinase of rabbit skeletal muscle - : Biochem J. 187

JE Park,(1992)



Archive Article

Cover Media Content

Volume : 23 / No. : 3 / Pub. : 2007-03
  1. Cecal Goblet Cell Response On Intracellular Development Of Eimeria Tenella In Susceptible And Infected Chickens
  2. Protein Characterization Of Snail Mucin (achasin) Local Isolate As An Antibacterial Factor
  3. Trypsin And Pronase Treatment On The Development Of Mouse Embryos Contaminated By K99 Escherichia Coli
  4. The Optimation Of Tyrosine Kinase Activity From Sperm Isolation Of Friesian Holstein Cow
  5. Insulin-like Growth Factor I (igf-i) Complex Seminal Plasma Of Goatratio Glicoprotein : Protein : Carbohydrate
  6. The Role Of Choline Esterase On Vesicle Brain Development Of Chicken Embryo Of Exposure By Carbofuran Insecticide
  7. Marker-sets For Paternity And Identity Test In Pigs By Meansmicrosatellite Analysis
  8. Bcg Vaccination Increased Roi Secretion In Dogs Macrophage Infected With Mycobacterium Tuberculosis
  9. Antifertility Effect Of Aqoueus Extract Betel Nut Of Areca Catechu: As An Apoptosis Agent On Testis Connective Cells Of Rattus Norvegicus
  10. The Morphology Of Mandibular And Lingual Glands Of Chicken (gallus Sp) And Quail (coturnix Coturnix): With Special Refference To The Distributionand Carbohydrate Content
  11. Detection Of Rabies Virus In Saliva And Brain Using Antibody From G Protein As Material Diagnostic By Indirect Double Antibody Sandwich Elisa Technique
  12. Study To Rice Brand In Drying Ensilage Product From Edible Snail For Decrease Higroscopies As Feed Stuff
  13. Potency Rice Straw Was Deammoniated And Fermented By Celulolytic Bacteria On The Dry Matter Consumption, Body Weight Gain And Feed Conversion Of Sheep
  14. Cecal Goblet Cell Response On Intracellular Development Of Eimeria Tenella In Susceptible And Infected Chickens
  15. Protein Characterization Of Snail Mucin (achasin) Local Isolate As An Antibacterial Factor
  16. The Role Of Choline Esterase On Vesicle Brain Development Of Chicken Embryo Of Exposure By Carbofuran Insecticide
  17. The Optimation Of Tyrosine Kinase Activity From Sperm Isolation Of Friesian Holstein Cow
  18. Insulin-like Growth Factor I (igf-i) Complex Seminal Plasma Of Goat Ratio Glicoprotein : Protein : Carbohydrate
  19. Marker-sets For Paternity And Identity Test In Pigs By Means Microsatellite Analysis
  20. Bcg Vaccination Increased Roi Secretion In Dogs Macrophage Infected With Mycobacterium Tuberculosis
  21. Antifertility Effect Of Aqoueus Extract Betel Nut Of Areca Catechu: As An Apoptosis Agent On Testis Connective Cells Of Rattus Norvegicus
  22. Trypsin And Pronase Treatment On The Development Of Mouse Embryos Contaminated By K99 Escherichia Coli
  23. The Morphology Of Mandibular And Lingual Glands Of Chicken (gallus Sp) And Quail (coturnix Coturnix): With Special Refference To The Distribution And Carbohydrate Content
  24. Detection Of Rabies Virus In Saliva And Brain Using Antibody From G Protein As Material Diagnostic By Indirect Double Antibody Sandwich Elisa Technique
  25. Study To Rice Brand In Drying Ensilage Product From Edible Snail For Decrease Higroscopies As Feed Stuff
  26. Potency Rice Straw Was Deammoniated And Fermented By Celulolytic Bacteria On The Dry Matter Consumption, Body Weight Gain And Feed Conversion Of Sheep
  27. Cecal Goblet Cell Response On Intracellular Development Of Eimeria Tenella In Susceptible And Infected Chickens

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