UNIVERSITAS AIRLANGGA



Detail Article

Media Kedokteran Hewan

ISSN 2015-8930

Vol. 18 / No. 1 / Published : 2002-04

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Original Article :

The separation of sex bearing chromosome sperm and embryo development produced of in vitro fertilization of madura cattle

Author :

  1. Laba Mahaputra*1
  2. Imam Mustofa*2
  1. Laboratorium Kebidanan Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya.
  2. Laboratorium Kebidanan Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya.

Abstract :

In an attempt to separate X and Y bearing chromosome spermatozoa using several techniques, a study has been designed to evaluate the effect of the treatment itself to the number of blastomeres of the embryos resulted 48 hours after an in vitro fertilization of these sperm to eggs. Frozen thawed Madura bull semen were treated in six different methods to separate X and Y bearing chromosome spermatozoa by using sephadex, percoll, Swim up , Swim up + Aside migration, direct current electricity 1,5Volt and control group. Ovaries were collected from an abattoir and subsequently oocytes were matured in a TCM199 media with a supplement of 10% estrous cow serum in a 5% C02 incubator for 24 hours. Fourty eight hours after the in vitro fertilization using the sperm collected from these separation methods, the percentage of embryos consisted of more than 4 blastomeres and that of less than 4 blastomeres were determined. The result showed that the effectiveness technique on separating the spermatozoa Y bearing chromosome were Swim up , combination of Swim up and Aside migration, and anode electricity 1,5 Volt (p

Keyword :

X Y sperm separation, IVF, Madura cattle,


References :





Archive Article

Cover Media Content

Volume : 18 / No. : 1 / Pub. : 2002-04
  1. The Effect Of Ultra Violet (uv) Radiation On Post Thawing Sperm Of Dairy Cattle Against Sex Ratio Of Sperm Cells Bearing Chromosome X And Y
  2. Equalion Models Of Progesterone And Estradiol Concentrations With Pregnancy Age In Ewes
  3. The Effect Of Antibodies Anti-inhibin In Obtaining Of Anti-idiotipic Antibodies On Mice
  4. The Separation Of Sex Bearing Chromosome Sperm And Embryo Development Produced Of In Vitro Fertilization Of Madura Cattle
  5. Chichen Embryo Fibroblast Cell Culture As An Media Hybrid B Cell Limphocyte And Mouse Myeloma Cell In Incubator Without Carbon-dioxyde
  6. Analysis Of The Chemically And Freeze Dried Preserved Complement Titer Stability
  7. Electron Microscope Scanning (ems) And Morphology Of The Some Species Of Haemonchus Sp. From Goat/sheep Slaughtered At Pegirian Slaughter House Surabaya
  8. Controlling Toxocariasis Infection On Calves In Madura Against Isolated The Volatile Oil From Curcuma Aeruginosa Roxb (temuireng)
  9. Analysis Of Restriction Fragment Length Polymorphisms For Molecular Epidemiological Tracing Of Escherichia Coli Strains Using Probes
  10. Morphofunction Evaluation Of Porcine Autogeneous Cephalic Vein Grafts In Arterial Circulation
  11. Estrogenicity Effect Test Of Piper Nigrum L. On Female Rat
  12. The Effect Of Frog Waste Meal In Feed On Abdominal Fat Percentage And Subcutan Fat Value Of Broiler Chicken
  13. Comparison Of Aeromonas Hydrophila Vaccination Method By Immersion And Hyperosmotic Infiltration Against The Antibody Titers

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